摘要
目的:研究舌鳞癌淋巴管生成及淋巴管密度/淋巴管相对面积(LVD/LVA)与颈淋巴结转移的关系,为术前准确判断颈淋巴结状况提供参考。方法:口腔颌面外科接受舌癌切除及颈淋巴清扫术的标本63例;对HE染色阴性的淋巴结,应用细胞角蛋白CK(AE1/AE3)标记检测微转移,以免疫组织化学检测结果判断淋巴结转移;以LYVE-1作为淋巴管内皮标志物,研究舌癌淋巴管生成参数——淋巴管密度(LVD)和淋巴管面积(LVA)与颈淋巴结转移及其他临床病理变量(年龄、性别、T分类、病理分级、浸润方式)之间的关系。应用SPSS10.0统计软件包对所得数据进行Mann-whitneyU检验。结果:应用细胞角蛋白CK(AE1/AE3)标记免疫组化法检测微转移,提高了淋巴结转移的检出率;舌癌实质内未见到LYVE-1+的脉管结构,LYVE-1+脉管多位于癌周,癌周LVD、LVA与颈淋巴结转移及其他临床病理变量均无关。结论:舌癌癌周淋巴管密度(LVD)、淋巴管面积(LVA)与颈淋巴结转移及其他临床病理变量均无关,不宜作为术前评估淋巴结状况的指标。
PURPOSE： To study the relationship between lymphatic vascular density（LVD） /relative lymphatic vascular area （LVA）and cervical lymph node metastasis in tongue squamous cell carcinoma. METHODS： 63 cases with tongue SCC underwent resection of primary lesion and neck dissection were recorded. Cytokerotin （CK） was applied as a marker for epithelial cells to detect micrometastatic lesions in the cervical lymph nodes. The special marker of lymphatic vessles, LYVE-1, was used to study the relationship between lymphatic vessel parameters （LVD/LVA） and cervical lymph nodes status and other clinicopathologic parameters,such as age, sex, T stage, histological degree of differentiation and pattern of invasion.SPSS10.0 software package was used for Mann-whitney U test. RESULTS： Immunohistochemistry,using CK,was capable of increasing the detection of micrometastasis in lymph nodes.No LYVE-1-positive intratumoral lymph vessels were present within SCC of tongue.Some dilated lymphatics were seen in the vicinity of the tumor.The peritumoral LVD/ LVA was not associated with cervical lymph node metastasis and other clinicopathologic parameters. CONCLUSIONS： The peritumoral lymphatic parameters （LVD/LVA） were not associated with cervical lymph node metastasis.
出处
《中国口腔颌面外科杂志》
CAS
2007年第1期24-27,共4页
China Journal of Oral and Maxillofacial Surgery
关键词
舌鳞状细胞癌
LYVE-1
淋巴管生成
转移
Squamous cell carcinoma of tongue
LYVE- 1
Lymphangiogenesis
Metastasis
