摘要
目的研究淡色库蚊对残杀威抗性与酯酶和乙酰胆碱酯酶的关系。方法WHO浸渍法检测蚊虫抗性指数;分光光度法检测羧酸酯酶和乙酰胆碱酯酶活性。结果使用残杀威对淡色库蚊野外品系进行筛选,筛选8代后抗性指数由原来的4.77倍上升至12.07倍。对敏感品系和抗性品系分别进行羧酸酯酶和乙酰胆碱酯酶活性测定,二者的羧酸酯酶活性差异无统计学意义(P>0.05),而二者的乙酰胆碱酯酶活性在高浓度残杀威抑制浓度(100×10-6、10×10-6、1×10-6)下,抑制率敏感品系分别为(93.92±0.65)%、(91.69±0.54)%和(87.03±1.14)%,抗性品系分别为(89.81±0.92)%、(87.41±1.66)%和(83.84±0.36)%,差异具有统计学意义(P<0.05)。结论羧酸酯酶在对残杀威的抗性形成中作用较小,而乙酰胆碱酯酶的敏感性降低与抗性品系的抗性形成有关。
Objective To study the activity of relationship between esterase, acetylcholinesterase and the resistance to propoxur in the larva of Culex pipiens pallens. Methods WHO bioassay and spectrophotometric method were used. Results A strain from field selected using propoxur in lab for generations with 12.07-fold resistance ratio was abtained, which the parent was 4.77-fold. The difference of esterase activity between sensitive and resistant strain was not significant. Otherwise, the acetylcholinesterase activity between two strains existed significant difference at high concentration （100×10^- 6, 10×10^-6 and 1×10^-6 ） propoxur. The inhibiting rate was （93.92 ±0.65 ） %, （91.69± 0.54） % and （87.03 ± 1.14） % respectively in sensitive strain, and （89.81±0.92） %, （87.41± 1.66） % and （83.84 ± 0.36） % respectively in resistant strain. Conclusion Metabolic detoxification induced by esterase play a minor role in propoxur-resistant strain. The insensitive AChE may be the main factor in related to the propoxur resistance.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2007年第1期9-11,共3页
Chinese Journal of Vector Biology and Control
关键词
淡色库蚊
残杀威
羧酸酯酶
乙酰胆碱酯酶
Culex pipiens pallens
Propoxur
Esterase
Acetylcholinesterase
