摘要
目的:观察大白鼠磨牙窝洞制备后,修复性牙本质形成过程中一氧化氮合酶NOS的表达。方法:大白鼠磨牙窝洞制备后制作冰冻切片,免疫组化染色观察成牙本质细胞与牙髓细胞中一氧化氮合酶的表达。结果:正常大白鼠磨牙中,内皮型一氧化氮合酶(eNOS)呈阳性反应,而诱导型一氧化氮合酶(iNOS)呈阴性反应。牙体制备后即刻,损伤的成牙本质细胞中两种一氧化氮合酶均呈弱阳性反应;制备1d后,在牙髓牙本质界中浸润的中性粒细胞中表现为诱导型一氧化氮合酶(iNOS)的强阳性反应,并在制备3d后,扩展至全部牙髓细胞;同阶段,内皮型一氧化氮合酶(eNOS)在上述细胞中,均呈弱阳性反应。制备7d后,修复性牙本质深部成牙本质细胞中,诱导型一氧化氮合酶(iN-OS)呈阴性反应,而内皮型一氧化氮合酶(eNOS)呈阳性反应。结论:一氧化氮参与了修复性牙本质形成过程中成牙本质细胞的分化与功能调节。
Objective：To Investigate the NOS expression in reparative dentinogenesis after tooth preparation. Method：The expression of NOSs in the odontoblasts and pulp cells was detected by immuno- histochemistry statining on crysection after the cavity prepartion on the mesial surface of the maxillary first molars of rats. Result： In normal rat molars, odontoblasts were endothelial NO synthase （eNOS）-positive,but inducible NOS （iNOS）-negative. Immediately after preparation, damaged odontoblasts were positive for both enzymes, iNOS was strongly observed in neutrophils after 1 day and in odontoblasts and whole pulp cells after 3 days. eNOS was expressed weakly in these cells after 1 to 3 days. After 7 days, they were negative for iNOS, but eNOS-positive odontoblasts were seen under the reparative dentin. Conclusion： These findings suggest that NO takes part in regulating odontoblasts in reparative dentinogenesis.
出处
《临床口腔医学杂志》
2007年第1期29-32,共4页
Journal of Clinical Stomatology
