摘要
目的:探讨ClC-3和NF-κB在膀胱移行细胞癌中的表达及意义。方法:应用免疫组织化学染色技术检测31例病理诊断为膀胱移行细胞癌和6例正常膀胱组织中ClC-3和NF-κB的表达。结果:膀胱移行细胞癌组织中ClC-3主要在癌细胞的胞膜表达,阳性表达率74%(23/31)。其中膀胱移行细胞癌Ⅰ级阳性表达率40%(4/10),Ⅱ级阳性表达率81%(9/11)、Ⅲ级阳性表达率100%(10/10)。NF-κB在癌细胞的胞浆内表达,阳性表达率83%(26/31)。其中膀胱移行细胞癌Ⅰ级阳性表达率60%(6/10),Ⅱ级阳性表达率90%(10/11),Ⅲ级阳性表达率100%(10/10)。6例正常膀胱组织中仅有1例ClC-3表达弱阳性,NF-κB表达均为阴性。膀胱移行细胞癌组织中ClC-3和NF-κB阳性表达率高于正常膀胱组织(P<0.01)。结论:ClC-3和NF-κB可能与膀胱移行细胞癌病理分级和增殖有关。
Objective To investigate the expressions of ClC-3 and NF-kB in transitional cell carcinoma of the bladder （TCCB） specimen and their significances. Methods The expressions of C1C-3 and NF-kB were detected in 31 cases of TCCB and 6 cases of normal bladder tissue as control by immunohistochemical staining. Results In the tissues of TCCB, ClC-3 was mainly located in the cell membrane, the positive rate of ClC-3 was 74% （23 /31）; the posivive rates of Ⅰ, Ⅱ and Ⅲ grades were 40% （4/10）, 81% （9/11）, and 100% （10/10）, respectively. NF-kB was mainly distributed in the cytoplasm, the positive rate of NF-kB was 83% （26/31） ; the positive rates of Ⅰ , Ⅱ and Ⅲ grades were 60% （6/10）, 90% （10/11） and 100~ （10/10）, respectively. Only one specimen showed weak positive staining, and negative staining of NF-kB was found in 6 cases of normal bladder tissues. The positive rates of ClC-3 and NF-kB in tissue of TCCB were higher than those in normal bladder tissue （P 〈0. 01）. Conclusion ClC-3 and NF-kB may be related to the pathological grade and proliferation of TCCB.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期135-137,F0003,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(20060271)
