摘要
目的:观察人胶质瘤细胞的体外侵袭能力及对胶原的溶解作用。方法:在体外用Boyden小室侵袭实验评价人恶性胶质瘤细胞株U87MG穿过Matrigel基膜胶的迁移能力;用琼脂-明胶凝胶观察U87MG细胞条件培养基对基质胶原溶解的影响。结果:U87MG胶质瘤细胞穿过Matrigel膜的细胞数明显多于加入EDTA或加入抗MMP-2抗体的U87MG胶质瘤细胞的穿膜细胞数(P<0.01),而后两者穿膜细胞数之间的差异无显著性(P>0.05),EDTA或抗MMP-2抗体对U87MG细胞的穿膜细胞数有明显的抑制作用,抑制率分别为79.2%和77.1%。U87MG细胞条件培养液能在凝胶孔周围引起明显增大的透明环,用EDTA或抗MMP-2抗体抑制MMP-2酶活性可明显减少透明环的形成。结论:U87MG胶质瘤细胞的侵袭能力和胶原降解作用取决于MMP-2,提示MMP-2在胶质瘤侵袭生长中起重要作用。
Objective To investigate the invasion ability and collagenolytic activity of human glioma cells in vitro. Methods Boyden chamber invasion assay was employed to evaluate the cell migration ability of human malignant glioma cell line U87MG in vitro and the effect of conditioned medium of U87MG cells on matrix collagenolytic activity was tested by agavgelatin gel. Results The number of U87MG glioma cells migrating through the Matrigel-coated membrane was more than that of addition of EDTA or anti MMP-2 antibody in U87MG glioma cells （P〈0.01）, and the difference between the numbers of the migrated cells in the latter two was not significant （P：〉 0.05）. EDTA or anti-MMP-2 antibody markedly inhibited the number of the migrated cells, the inhibitory rates were 79.2 % and 77.1 N, respectively. The conditioned medium collected from the U87MG cells showed an increase in the transparent ring area on agar-gelatin gel. Inhibition of MMP 2 enzymatic activity by EDTA or anti MMP 2 antibody reduced the transparent ring area on agar-gelatin gel. Conclusion Both invasion ability and collagenolytic activity of U87MG cells are depended on MMP2, suggesting that MMP-2 plays an important role in glioma invasiveness.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期84-87,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(20030423-03)
关键词
胶质瘤细胞
侵袭能力
胶原降解
明胶酶A
human glioma cell
invasion ability
collagen degradation
gelatinase A
