摘要
目的:克隆新的与激活素受体ⅡA(ActRⅡA)相互作用蛋白,即激活素受体相互作用蛋白4(ActRIP4)基因。方法:应用ActRIP2基因片段作为探针,从小鼠cDNA文库中钓取ActRIP4 cDNA;采用哺乳细胞双杂交进一步确定ActRIP4与ActRⅡA的相互作用;RT-PCR检测ActRIP4 mRNA在组织中的表达情况。结果:克隆的ActRIP4基因全长691bp,ORF区编码118个氨基酸残基;ActRIP4与ActRⅡA具有特异性结合作用;天然的ActRIP4 mRNA在小鼠多种组织中表达。结论:ActRIP4属于ActRIP家族新成员,可以与ActRⅡA相互作用,可能在多种组织中介导激活素受体信号传导过程。
Objective To clone the gene of a novel aetivin receptor-interacting protein 4 （AetRIP4） which interacts with type Ⅱaetivin receptor （AetRⅡ A） . Methods The ActRIP4 gene from a mouse eDNA library was cloned by using the AetRIP2 gene fragment as the probe. By mammalian cell two-hybrid, its ability to combine with AetR Ⅱ A was confirmed. The expressions of AetRIP4 mRNA in tissues were analyzed by RT-PCR. Results The cloned fulllength eDNA for AetRIP4 was 691bp, which ORF was encoding 118 amino acids. The specific mammalian cell two- hybrid analysis revealed that AetRIP4 could specifically combine with AetR Ⅱ A. The results of RT-PCR showed that ActRIP4 mRNA expressed in many tissues. Conclusion AetRIP4 is a novel member of ActRIP family, and has the ability to specifically combine with ActR ⅡA, which may mediate signaling transduction of activin receptor in various tissues.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期37-40,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(304004003044004430571688)
关键词
激活素
受体相互作用蛋白
哺乳动物细胞双杂交
逆转录聚合酶链反应
activin
receptor-interacting protein
mammalian cell two-hybrid
reverse transcriptase polymerase chain reaction
