摘要
目的:建立一种测定人体血浆中吲哒帕胺血药浓度的高效液相色谱法。方法:采用 ULTRON VX-ODS 色谱柱(5μm,4.6 mm×250 mm),以甲醇-乙腈-水(50∶5∶45)为流动相,流速1 mL·min^(-1),柱温:室温,检测波长242 nm。结果:本方法线性范围为0.54～172.8ng·mL^(-1),r=0.9940,方法定量限为(0.54±0.04)ng·mL^(-1)(S/N>10),方法回收率为97.8%～108.1%(n=15),日内 RSD 为3.0%～13.4%(n:5),日间 RSD 为2.5%～14.4%(n=5).结论:本方法灵敏度高,操作简便准确,可用于人体血浆中吲哒帕胺浓度的测定及药动学研究.
Objective： To establish an RP - HPLC method for the determination of indapamide in human plasma. Methods：Chromatography was conducted on an ULTRON VX - ODS column（5μm,4. 6 mm × 250 mm） at room temperature and detected at 242 nm. The mobile phase was methanol -acetonitrile -water（50：5：45 ）at a flow rate of 1 mL ·min^-1. Results：The linear range of method was 0. 54 - 172. 8 ng ·mL^-1 ,r =0. 9940. The limit of quanti- fication was 0. 54 ng ·mL^-1 ;The method recovery was 97. 8% - 108. 1% （n = 15）. Within - day precision（RSD） was 3.0% - 13.4 % （ n = 5 ）, between - day precision （ RSD ） was 2. 5 % - 14. 4% （ n = 5 ）. Conclusion： This method is sensitive, simple, accurate and applicable to study of pharmacokinetics and bioavailability of indapamide.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第1期69-71,共3页
Chinese Journal of Pharmaceutical Analysis
