摘要
在2个单级悬浮床生物反应器中,以双酶法制备的玉米粉糖化液为底物,进行了废液循环条件下自絮凝颗粒酵母乙醇连续发酵与清液发酵对比实验。对于废液循环实验,每隔5d将收集到的发酵液集中精馏处理,得到的废糟液直接用于玉米粉调浆。实验结果表明,在稀释速率0.05 h-1,废糟液全循环条件下,装置达到稳定状态后,乙醇、残还原糖、无机磷、无机氮、蛋白和总固型物浓度分别稳定在9.4%(v/v)、26 g/L、11 g/L、3.36 g/L、0.136 g/L和85 g/L左右;乙醇浓度与对照组相差不大,但残还原糖、无机磷及总固形物在废糟液循环使用的初期出现积累,但随运行时间的延长而趋于稳定,说明对酵母生长和乙醇发酵没有产生明显影响。
Continuous ethanol fermentations suspended- bed bioreactors, respectively, using with and without recycling of distillage were carried out in two two- stage enzymatic hydrolyte of corn powder as medium. For the fermentation coupled with distillage recycle, every five days, the total backset distillage collected was directly used to mix the corn powder, and the experimental results revealed that the concentrations of ethanol, residual reductive sugar, inorganic phosphate and total solids in the broth were maintained stably around 9.4% （v/v）, 26 g/ L, 11 g/L, 3.36 g/L, 0. 136 g/L and 85 g/L, respectively. Compared with the fermentation without distillage recycle, accumulations of residual reductive sugar, inorganic phosphate and total solids were observed in the beginning period of fermentation. But a steady- state was reached after a long time running, and the ethanol concentration was closed to that of the control, which indicated that these accumulations did not exert significant inhibition on cell growth and ethanol formation.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2006年第12期24-27,共4页
Food and Fermentation Industries
基金
国家863高科技研究发展计划项目(2002AA647060)
关键词
自絮凝酵母
乙醇连续发酵
废糟液全循环
总固形物
self-flocculating yeast, continuous ethanol fermentation, distillage recycling, total solids
