摘要
为了研究干扰素α对白血病细胞U937細胞的抗增殖作用及其机制,用不同浓度的干扰素α(500U/L、1000U/L、2000U/L、3000U/L、4000U/L)对U937細胞作用不同时间(0、12、24、36和48小时),用MTT法检测细胞生长抑制率,应用流式细胞术(FCM)检测细胞凋亡率,RT-PCR方法分析cyclinEmRNA的表达。结果表明:不同浓度的干扰素α处理细胞后,U937细胞生长明显受抑,2000U/L干扰素α能引起细胞凋亡,凋亡率为25.28%-70.54%(P<0.01),细胞周期相关基因cyclinEmRNA表达降低;干扰素α对U937细胞的抑制率,呈浓度-时间依赖性。结论:干扰素α对U937细胞有明显抗增殖和促凋亡作用,此结果将为干扰素-α在白血病临床治疗方面的应用提供有力的实验证据。
This study was aimed to investigate the anti-proliferation effect of interferon-α （IFN-α） on leukemic U937 cells and its mechanism. The U937 cells were given with various concentrations of IFN-α（500, 1 000, 2 000, 3 000 and 4 000 U/L） and at different time （0, 12, 24, 36, 48 hours）, the inhibitory ratio was measured by MTT assay, apoptosis rate was detected by flow cytometry（ FCM）, the expression of cell cycle-associated cyclin E mRNA was measured by RT-PCR. The results showed that IFN-α （2 000 U/L） could cause apoptosis, after being treated by various concentrations of IFN-α, the growth of U937 cells was inhibited significantly, the apoptosis rate was 25.82% - 70.54% （ P 〈 0.01 ）, the cycle-associated cyclin E mRNA expression decreased, the growth of U937 ceils was significantly inhibited, the suppression of U937 by IFN-α was both in time-and dose-dependent manner. It is concluded that IFN-α has apparent anti-proliferation and apoptosis-inducing effects on U937 cells. These results will provide strong laboratory evidence for IFN-α clinical application in leukemia therapy.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第1期52-55,共4页
Journal of Experimental Hematology
