摘要
One kind of novel BLMs was fabricated by patch-clamp pipette technology characterized in considerably sensitive to changes of electrochemical parameters.Detectiye currents and voltage presented linear relationship when BLMs was formed and it could be confirmed by Gramicidin method.Ion current was increased by dihexyl (C_ (12)) modified ssDNA fixed on the BLMs and also indicated linear relationship to ssDNA's concentration due to the interaction of (C_ 12)-ssDNA and BLMs.Further more,the regression equations were different from BLMs fixed with ssDNA probe and a blank control BLM in the same experimental conditions.The ssDNA probe was successfully fixed on patch-clamp pipette supported-BLMs.Based on our studies,a biosensor with reactive element of patch-clamp pipette-supported BLMs has been established.
One kind of novel BLMs was fabricated by patch-clamp pipette technology characterized in considerably sensitive to changes of electrochemical parameters. Detectiye currents and voltage presented linear relationship when BLMs was formed and it could be confirmed by Gramicidin method. Ion current was increased by dihexyl（C12） modified ssDNA fixed on the BLMs and also indicated linear relationship to ssDNA＇s concentration due to the interaction of C12-55DNA and BLMs. Further more, the regression equations were different from BLMs fixed with ssDNA probe and a blank control BLM in the same experimental conditions. The ssDNA probe was successfully fixed on patch-clamp pipette supported-BLMs. Based on our studies, a biosensor with reactive element of patch-clamp pipette-supported BLMs has been established.
出处
《稀有金属材料与工程》
SCIE
EI
CAS
CSCD
北大核心
2006年第A03期324-326,共3页
Rare Metal Materials and Engineering
