摘要
建立了反相高效液相色谱法测定贯叶连翘中氨基酸含量的方法。采用BDS HYPERSIL C18色谱柱(250 mm×4．6 mm,5μm),流动相(A):0．02mol／L醋酸钠(pH 7．2)十三乙胺+四氢呋喃;流动相(B):0．02mol／L醋酸钠(pH 7．2)+乙腈+甲醇,流速0．6 mL／min,柱温35℃,检测波长338nm。测定结果具有良好的线性(相关系数0．9874-0．9995)和重复性(相对标准偏差<4．89%),回收率91％～107％。方法简便实用,实际样品分析结果令人满意。
A RP-HPLC method for the determination A BDS Hypersil C18 column （250mm×4.6mm, 7.2）-triethylamine-THF and B of 0.02 mol/L of amino acid in Hypericum perforatum was established. 5μm） was used with solution A of 0.02mol/L NaAc （pH NaAc （pH 7.2）-acetonitrile-methanol as mobile phases.The flow rate was 0. 6ml/min, column temperature 35℃ and detection at 338 nm. The results showedgood linear relationship （r = 0.9874～ 0.9995 ） and repeatibility （RSD〈 4.89 % ）.The recovery was 91%～107%. The method is simple and practical and the samples were determined with satisfaction.
出处
《分析仪器》
CAS
2007年第1期27-30,共4页
Analytical Instrumentation
