摘要
目的:检测5株人胰腺癌细胞株Hedgehog信号转导通路成员锌指转录因子GLI1、PTCH1基因的表达情况,测定并分析其基因序列。方法:用RT-PCR方法检测5株胰腺癌细胞株及25例正常胰腺组织GLI1、PTCH1 mRNA的表达;纯化、回收目的基因GLI1、PTCH1,将其分别插入克隆载体pTA2,限制性内切酶酶切鉴定,测定序列。结果:5株胰腺癌细胞株均表达GLI1、PTCH1,25例正常胰腺组织均不表达GLI1、PTCH1;成功构建重组质粒pTA2/GLI1、pTA2/PTCH1,测序结果与GeneBank公布的基因序列一致。结论:Hedgehog信号转导通路的过度激活参与了胰腺癌的发生,可能是胰腺癌治疗的潜在新靶标。
Objective To investigate the expression of glioma-associated oncogene homolog 1 （GLI1）and patched homolog 1 （PTCH1）in human pancreatic cancer cell lines, and analyze their sequences. Methods RT-PCR was used to detect the mRNA expression of GLI1 and PTCH1 in 5 pancreatic cancer cell lines and 25 normal pancreatic tissues. The products of RT-PCR were purified and cloned into pTA2 vectors, then identified by enzyme digestion and DNA sequencing. Results Both GLI1 and PTCH1 mRNA were expressed in all 5 pancreatic cancer cell lines. Yet, there were no GLI1 and PTCH1 mRNA expression in normal pancreatic tissues. Recombinant pTA2/GLI1 and pTA2/PTCH1 were successfully constructed. The results of sequencing were identical with those in GeneBank. Conclusions The activation of hedgehog signal pathway might participate the tumorigenesis of pancreatic cancer, and could be served as a potential therapeutic target.
出处
《内科理论与实践》
2007年第1期52-55,共4页
Journal of Internal Medicine Concepts & Practice
