摘要
BACKGROUND： Postoperative cognitive dysfunction （POCD） is very common in elderly patients. Some Chinese agents for eliminating oxygen-derived free radicals and reducing oxidative stress injury can obviously decrease the error times of the animal models of Alzheimer disease in the water maze test. However, whether they can be used to treat POCD or not is unclear. OBJECTIVE： To observe the changes of learning and memory abilities and the levels of intedeukin-6 （IL-6） and tumor necrosis factor alpha （TNF-α） in aging rats after ketamine-induced anesthesia, and investigate the effects of Fufang Shexiang injection which can eliminate oxygen-derived free radicals. DESIGN ： A randomized controlled animal study SETTING ： Experimental animal center of Guangzhou University of Traditional Chinese Medicine MATERIALS ： Twenty-eight SD aging rats （20 months old, 350-400 g, male or female） randomly divided into 4 groups with 7 rats in each group： control group, ketamine group, ketamine＋low-dosage Fufang Shexiang group and ketamine＋high-dosage Fufang Shexianggroup. Ketamine （Shanghai Zhongxi Pharmaceutical Co., Ltd., China, 50 mg/mL, 2 mL/ampule, Batch No. 040207）; Fufang Shexiang injection （Jiling Weiwei Pharmaceutical Co., Ltd., China, 2 mL/ampule, Batch No. 20031203） was composed of musk, curcumae, camphol, acon graminei rhizome, agastache rugosa, menthol, bezoar, comu bubali, scutellaria, gardenia, oncha margaritifera usta, etc. Enzyme-linked immunoabsorbent assay （ELISA） kit （Jingmei Bio-Tech Co., Ltd, Beijing, Batch No. 20050915）. Morris water maze （Upwards Teksystems Co.,Ltd., Beijing）. METHODS ： The experiment was carded out in the experimental animal center of Guangzhou university of Traditional Chinese Medicine From September to October in 2005. （1） Grouping and interventions： In the control group, the rats were intraperitoneally injected with saline （4 mL/kg per day） at 1-6 days; In the ketamine group, ketamine＋low and high-dosage Fufang Shexiang groups
BACKGROUND： Postoperative cognitive dysfunction （POCD） is very common in elderly patients. Some Chinese agents for eliminating oxygen-derived free radicals and reducing oxidative stress injury can obviously decrease the error times of the animal models of Alzheimer disease in the water maze test. However, whether they can be used to treat POCD or not is unclear. OBJECTIVE： To observe the changes of learning and memory abilities and the levels of intedeukin-6 （IL-6） and tumor necrosis factor alpha （TNF-α） in aging rats after ketamine-induced anesthesia, and investigate the effects of Fufang Shexiang injection which can eliminate oxygen-derived free radicals. DESIGN ： A randomized controlled animal study SETTING ： Experimental animal center of Guangzhou University of Traditional Chinese Medicine MATERIALS ： Twenty-eight SD aging rats （20 months old, 350-400 g, male or female） randomly divided into 4 groups with 7 rats in each group： control group, ketamine group, ketamine＋low-dosage Fufang Shexiang group and ketamine＋high-dosage Fufang Shexianggroup. Ketamine （Shanghai Zhongxi Pharmaceutical Co., Ltd., China, 50 mg/mL, 2 mL/ampule, Batch No. 040207）; Fufang Shexiang injection （Jiling Weiwei Pharmaceutical Co., Ltd., China, 2 mL/ampule, Batch No. 20031203） was composed of musk, curcumae, camphol, acon graminei rhizome, agastache rugosa, menthol, bezoar, comu bubali, scutellaria, gardenia, oncha margaritifera usta, etc. Enzyme-linked immunoabsorbent assay （ELISA） kit （Jingmei Bio-Tech Co., Ltd, Beijing, Batch No. 20050915）. Morris water maze （Upwards Teksystems Co.,Ltd., Beijing）. METHODS ： The experiment was carded out in the experimental animal center of Guangzhou university of Traditional Chinese Medicine From September to October in 2005. （1） Grouping and interventions： In the control group, the rats were intraperitoneally injected with saline （4 mL/kg per day） at 1-6 days; In the ketamine group, ketamine＋low and high-dosage Fufang Shexiang groups
