摘要
目的:研究VEGF,b-FGF诱导下人脐血单个核细胞向血管内皮祖细胞(EPCs)分化.方法:从新鲜脐血中用Ficoll密度梯度离心法获取单个核细胞,在加有VEGF,bFGF的M199培养基中培养,培养7d,免疫组化(SABC法)和免疫荧光检测细胞表面抗原的表达.结果:人脐血血单个核细胞(MNCs)经体外VEGF,b-FGF的诱导分化,表达内皮祖细胞的表面标志KDR,CD1133和CD34.结论:人脐血单个核细胞在一定的培养条件下可诱导分化为内皮祖细胞.
AIM： To research a way for mononuclear cells from human umbilical cord blood in vitro to be differentiated into endothelial progenitor cells under the induction of VEGF and b-FGF. METHODS： The monanuelear cells were obtained from human umbilical cord blood by Ficoll density-gradient centrlfugation. The cells were suspended in medium 199 （M199） supplemented with VEGF, b-FGF for culture, differentiation, proliferation and identification. The expressions of specific antigens on cell surface were analyzed by immunohistochcmistry and immunofluorescence. RESULTS： The mononuclear cells from human peripheral blood which were cultured and induced in vitro by VEGF and b-FGF were positive for KDR, CDl133 and CD34. CONCLUSION： Mononuclear cells from human umbilical cord blood can be induced to differentiate into endothelial progenitor cells in vitro under certain condition.
出处
《第四军医大学学报》
北大核心
2007年第2期137-139,共3页
Journal of the Fourth Military Medical University
关键词
胎血
内皮祖细胞
诱导
fetal blood
endothelial progenitor cells
induction
