摘要
目的:研究肝卵圆细胞增殖模型大鼠Notch1,Jag-ged1mRNA表达的变化及苦参碱对其的影响.方法:雄性SD大鼠48只按体重随机分为4组:模型组(A组);苦参碱小剂量(B组);苦参碱组大剂量(C组);D组即正常对照组.用免疫组化方法观察造血干细胞标记(Thy-1)表达的变化、半定量RT-PCR方法观察肝组织中Notch1,Jagged1mRNA表达的变化.结果:与D组比较,A组Thy-1蛋白,Notch1mRNA,Jag-ged1mRNA表达显著升高(P<0.01),与A组比较,C组Thy-1蛋白,Notch1mRNA,Jagged1mRNA表达显著下调(P<0.01).结论:苦参碱可有效的调节Notch信号通路、下调Thy-1蛋白表达,可能是其抑制卵圆细胞过度增殖、诱导卵圆细胞向肝细胞方向定向分化的机制之一.
AIM： To study the expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats and the effect of matrine on them. METHODS： Forty-eight male SD rats were randomly separated into 4 groups according to body mass： model group（ group A） ,low-dose matrine group（ group B, 5 mg/100g）, high-dose matrine group （group C, 25 rag/100 g） , control group （group D）. The expression of Thy-1 protein was detected by enzyrnehistochemisty, and the expressions of Notch1, Jagged1 mRNA were detected by semi-quantitative RT-PCR method. RESULTS： Compared with group D , the expressions of Thy-1 protein, Notch1 mRNA, Jagged1 mRNA in group A were singnifieantly increased （P 〈 0.01 ）. Compared with group A, the expressions of Thy-1 protein, Notch1 mRNA,Jagged1 mRNA in group C were singnificantly increased （ P 〈 0. 01 ）. CONCLUSION： Matrine could regulate Notch signaling, decrease the expression of Thy-1 protein ,which might be one of the mechanisms of inhibiting the proliferation of hepatic oval cells and inducing rat hepatic oval cells to differentiate into mature hepatocytes.
出处
《第四军医大学学报》
北大核心
2007年第2期122-125,共4页
Journal of the Fourth Military Medical University
