摘要
目的建立清肝片的质量标准。方法采用薄层色谱法鉴别板蓝根、菌陈和甘草；采用高效液相色谱法测定样品中甘草酸的含量，高效液相的色谱条件为：Agilent Eclipse XDB-C18柱（4．6mm×250mm，5μm），流动相为甲醇-0．2mol·L^-1醋酸铵溶液-冰醋酸（67：33：1）。结果薄层鉴别专属性强，高效液相色谱法甘草酸铵在0．36-1．80μg范围内呈良好的线性关系，平均回收率98．33％。RSD为1．24％。结论该方法可更好地控制清肝片的质量。
Objective To establish the quality standard of Qinggan Tablet. Methods TLC was used to identify Radix lsatidis, Herba Artemisiae Scopariae and Radix Glycyrrhizae; and HPLC was used to determine the content of Glycyrrhiziate acid. Agilent Eclipse XDB -C18 column（4, 6 mm× 250 mm ,5 μm） with mobile phase of methanol- 0.2 mol · L^-1 ammonium acetate solution - glacial acetic acid（67： 33： 1 ） was used for the determination of puerarin. Results The TLC for identification was special and the linear range of puerarin was 0.36 - 1.80μg and recovery rate was 98.33% , RSD was 1.24%, respectively. Conclusion This method can be used to control the quality of Qinggan Tablet better.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第1期140-141,共2页
Lishizhen Medicine and Materia Medica Research
