摘要
目的观察nm23-H1基因在人原发性肝细胞癌中的突变情况,探讨nm23-H1基因突变与肝癌发生、发展及转移的关系。方法采用PCR-SSCP方法对16例肝癌组织、12例癌旁组织和4例正常肝组织的nm23-H1基因的第1、2、4外显子进行突变检测。结果共有5例发生nm23-H1基因突变。在1例癌旁组织中检测到nm23-H1基因第2外显子纯合缺失;在1例肝癌组织中第1外显子、1例第2外显子,癌旁组织中2例第2外显子检测到基因突变。结论nm23-H1基因突变在肝癌组织中发生率低,nm23-H1基因突变可能与肝癌进展及转移有关。
Objective： To investigate the mutation of nm23 - HI gene and to explore the relationship between nm23 - H1 mutation and the development, progression and metastasis of human hepatocellular carcinoma （HCC）. Methods： Mutations of the exonl, 2, 4 of nm23 -H1 gene in 16 HCC tissues, 12 adjacent liver tissues and 4 normal liver tissues were examined by means of PCR - SSCP. Results： Altogether there were 5 cases of nm23 - H1 gene mutation. One of exon 2 homozygous loss of allele of nm23 - H1 gene was examined in adjacent liver tissue. The nm23 - H1 gene mutation was also examined that one of exonl in HCC tissues, one of exon2 in adjacent liver tissue and two of exon2 in adjacent liver tissue. Conclusion： The mutation of rim23 - HI gene is rare in HCC. It is showed that nm23 - H1 gent mutation maybe associated with the progression and metastasis of HCC.
出处
《中国优生与遗传杂志》
2007年第1期23-24,88,共3页
Chinese Journal of Birth Health & Heredity
