摘要
目的通过研究共轭亚油酸(conjugated linoleic acid,CLA)对饮食诱导胰岛素抵抗大鼠葡萄糖运载体4(glucose transporter 4,GLUT4)蛋白表达的影响,探讨 CLA 抗糖尿病作用的机制。方法选用雄性 Wistar 大鼠,用随机数字表法按体重随机分为对照绀、高脂组、高脂+CLA 组(每100g 饲料含 CLA 分别为0.75 g、1.50 g、3.00 g),每组动物10只,观察 CLA 对胰岛素抵抗大鼠胰岛素、血糖水平的影响,并应用 Western blot 方法检测大鼠骨骼肌 GLUT4蛋白的表达水平。结果高脂组大鼠血清胰岛素和糖血水平分别为(11.11±2.73) μU/ml、(5.09±0.66)mmol/L,CLA 可降低肥胖大鼠的高胰岛素、高糖血症,低、中、高剂量组胰岛素水平分圳为(6.99±1.77) μU/ml、(7.36±1.48)μU/ml、(7.85±1.60) μU/ml,血糖水平分别为(4.28±0.72)mmoL/L、(4.18±0.55)mmol/L、(4.06±0.63)mmol/L,且高脂组大鼠骨骼肌 GLUT4蛋白的表达水平较基础组降低,CLA 可增加高脂组大鼠骨骼肌 GLUT4蛋白的表达水平。结论 CLA 可通过增加胰岛素抵抗大鼠骨骼肌 GLUT4蛋白的表达水平,改善胰岛素抵抗。
Objective To study the effects of conjugated linoleic acid （CLA） on expression of glucose transporter 4 （ GLUT4 ） protein in skeletal muscle of insulin resistant rat, and explore the mechanism of resisting diabetes by CLA. Methods Male Wistar rats were randomly separated into control group, highfat group and high fat plus CLA group（0.75 g% ,1.50 g% ,3.00 g% by deit weight）, and the effects of CLA on blood glucose and insulin levels of insulin resistant rat were observed , by using Western blot technique to measure the expression level of GLUT4 protein in skeletal muscle of insulin resistant rat. Results The serum insulin and glucose levels of obese rats were （ 11.11 ± 2.73 ） μU/ml, and （5.09 ± 0. 66） mmol/L,the supplement of CLA might decrease the hyperinsulinemia and hyperglycemia, and in CLA groups （0. 75 g%, 1.50 g%, 3.00 g% by deit weight） the serum insulin was （ 6. 99 ± 1.77 ） μU/ml, （7. 36 ±1.48）μU/ml and （7. 85±1.60） μU/ml （ P 〈 0. 05 ）, and the glucose levels were （4. 28 ± 0. 72 ） mmol/L, （4. 18 ±0.55） mmol/L（P 〈0.05）, （4.06± 0.63） mmol/L（P 〈 0.05） respoctively. The expression of GLUT4 protein in skeletal muscle of rat fed with high fat diet were decreased as compared with those fed with basic deit , and CLA might increase the expression of GLUT4 protein in skeletal muscle fed with high fat diet. Conclusions CLA improve the insulin resistance of obese rat, possibly acting through increasing the expression of GLUT4 protein in skeletal muscle of rat fed with high fat diet.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2007年第1期25-28,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金(30271118)
关键词
单糖转运蛋白质类
胰岛素抗药性
亚油酸类
Monosaccharide transport proteins
Insulin resistance
Linoleic acids
