摘要
采用三抗体夹心法（TAS-ELISA）研究了烟草漂浮苗中烟草花叶病毒（Tobacco mosaic virus，TMV）通过剪叶操作传播的特点。试验表明。剪一次TMV新鲜病叶的带毒剪刀连续剪10棵健康烟苗，剪叶后第30d花叶病的平均发病率为12．7％。苗盘试验表明，每剪1盘苗后用漂白粉消毒剪刀1次的情况下，在每盘苗加入6株TMV病苗，剪刀剪叶1次，剪叶后第10d平均发病率为13．0％-18．3％，发病株数增加3．5-4．9碚，无症烟苗移栽后15d。花叶病发病率可达65％-80％。在第1次剪叶前7d，每盘苗挑选6株用稀释1．0×10^-6倍的TMV病叶汁液摩擦接种，第1次剪叶后13d（接种后20d）留下病苗和拔走病苗处理带毒率分别为57．8％和37．8％。第3次剪叶后13d（接种后47d）带毒率都达到95．6％。在存在病株的情况下，剪叶传播烟草普通花叶病的效率很高。人工气候箱中，剪叶接种1．0×10^-6TMV病叶汁液，接种后11-12d TAS-ELISA可检测出阳性，接种后20—24d表现花叶症状。
In this paper, the spread character of Tobacco mosaic virus through clipping in tobacco float system was evaluated by TAS-ELISA method. The test results showed that：Among 10 tobacco seedlings which clipped by a clipper carried TMV through clipping a mosaic leaf, 12. 7% seedlings showed mosaic symptom after 30 d. After added 6 mosaic seedlings in a tray, clipped once, the average incidence of mosaic could reach to 13.0% - 18. 3%, about 65% - 80% symptomless seedlings became mosaic after transplanted 15 d. Seven days before first clipping, inoculated 6 seedlings in a tray with 1.0 × 10^-6 mosaic leaf juice, the seedling TMV-positive percentage of keep mosaic seedling treatment and move mosaic seedling treatment could reach to 57. 8% and 37. 8% respectively at 13th day after first clipping, both treatment seedling TMV-positive percentage could reach to 95.6% at 13th day after third clipping. In artificial climate chamber, by inoculated with 1.0 × 10^-6 mosaic leaf juice through clipping, TAS-ELISA could detect positive seedlings at 11 - 12 d, seedlings show mosaic symptom at 20 -24 d.
出处
《云南农业大学学报》
CAS
CSCD
2007年第1期45-48,60,共5页
Journal of Yunnan Agricultural University
基金
云南省烟草公司基金项目(04A15)
关键词
烟草
漂浮育苗
烟草花叶病毒
tobacco
floating seedling system
tobacco mosaic virus
