摘要
目的:分析DNA修复基因着色性干皮病基因DLys751Gln基因型频率和等位基因频率在中国汉族(四川泸州)和泰国人群中的分布特点,并比较其在两个群体中的分布有无差异。方法:实验于2006-01进行。选择150名无血缘关系的泸州医学院附属医院健康体检者,均为汉族,25～65岁,平均45岁;140名泰国无血缘关系个体,均来自曼谷地区,年龄25～63岁,平均46岁。采用聚合酶链反应-限制性片段长度多态性、聚丙烯酰胺凝胶电泳银染显色方法检测所有受试者的Lys751Gln等位基因频率和基因型频率。采用Promega公司的统计学软件PowerStats进行Hardy-Weinberg平衡检测,应用χ2检验或Fisher确切概率法分析Lys751Gln等位基因及基因型频率在中国汉族、泰国及其他人群中的分布是否有差异,采用SPSS11.5软件完成。结果:290名受试者全部进入结果分析。①基因型Lys/Lys,Lys/Gln和Gln/Gln在泸州汉族人群中的频率分别为72.67%,27.33%和0.00%,在泰国人群中的频率分别为87.86%,12.14%和0.00%。②基因型频率分布符合Hardy-Weinberg平衡定律,采用χ2检验或Fisher确切概率法进行统计分析,两个人群中基因型频率和等位基因频率分布有显著性差异(P<0.05)。结论:Lys751Gln基因多态性在中国汉族(四川泸州)和泰国人群中的分布有显著性差异,其多态性分布存在地域和种族差异。
AIM： To study on the allelic frequencies and genotypes frequencies of DNA repair gene xeroderma pigmentosum complementation group D （XPD） Lys751GIn in Chinese Han （Luzhou, Sichuan Province） and Thai populations, and explore the difference of the distribution between the two populations. METHODS： The experiment was conducted in January 2006. 150 Han unrelated healthy examinees aged 25-65 years with mean age of 45 years in Affiliated Hospital of Luzhou Medical Collage were selected; meanwhile, 140 Thai unrelated individuals from Thailand aged 25-63 years old with mean age of 46 years ware selected. Their allelic frequencies and genotypes frequencies ware identified by the polymerese chain reaction-restriction fragment lengthy polymorphism procedure, and polyacrylamide gel electrolphoresis and silver staining. The PowerStats from Promega Company was adopted for Hardy-Weinberg equilibrium detection; the difference in Lys751GIn allelic frequencies and genotypes frequencies between Han and Thai populations was analyzed by x^2-test or Fisher exact probability with SPSS 11.5 software. RESULTS： All the 290 subjects ware involved in theresult analysis. ①The genotypes frequencies of Lys/Lys, Lys/GIn and Gin/Gin in Luzhou Han populations ware 72.67%, 27.33% and 0.00% respectively, and in Thai ware 87.86%, 12.14% and 0.00% respectively. ②The distributions of genotypes frequencies in two populations were in accordance with Hardy-Weinberg eduUibdum and there ware significant differences in genotypes frequencies distributions among Luzhou Han and Thai populations analyzed with Chi-square test or Fisher exact probability （P 〈 0.05）. CONCLIUSION： The genotypes frequencies distributions of XPD Lys751GIn is different in Chinese Han （Luzhou, Sichuan） and Thai populations, which show specific ethnicity and geographic region.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第17期3210-3212,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
泸州医学院科研基金资助项目(435)~~
