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维生素C及E联合作用对体外大鼠胚胎中脑神经细胞分化和增殖的影响 被引量:9

Influences of vitamin C,vitamin E and the combination of vitamin C and E on the differentiation and proliferation of embryonal midbrain neurocytes of rats in vitro
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摘要 目的:观察维生素C,维生素E和维生素C+维生素E联合后对胚胎中脑神经细胞生长发育的影响。方法:实验于2006-03/04在江苏大学医学院研究中心细胞培养室完成。采用16d大鼠胚胎中脑神经细胞体外培养方法,观察不同剂量的维生素C(5,10,25,50μmol/L),维生素E(10,25,50,100μmol/L)和维生素C、维生素E联合作用(维生素C25μmol/L+维生素E50μmol/L,维生素C50μmol/L+维生素E100μmol/L),培养10d后收集细胞,并利用图像分析细胞形态的变化、蛋白质、丙二醛含量及超氧化物歧化酶活性指标。结果:①维生素C、维生素E和维生素C+维生素E联合能促进体外培养中脑神经细胞突起生长,集落数增多。②与正常对照组比较,维生素C10,25μmol/L组、维生素E10,25,50μmol/L组、维生素C25μmol/L+维生素E50μmol/L组神经细胞总蛋白相对含量明显增加。③与正常对照组比较,维生素C10,25μmol/L组、维生素E25,50μmol/L组、维生素C25μmol/L+维生素E50μmol/L组神经细胞超氧化物酶活性增加,丙二醛含量降低。④维生素C50μmol/L组、维生素E100μmol/L组和维生素C50μmol/L+维生素E100μmol/L组超氧化物酶活性低于正常对照组,丙二醛含量高于正常对照组。结论:维生素C、维生素E和维生素C+维生素E联合剂量在一定范围内能够明显提高中脑神经细胞的抗氧化能力,同时能促进胚胎中脑神经细胞分化和增殖作用。 AIM: To observe the effects of vitamin C (VC), vitamin E (VE) and the combination of VC and VE on rat embryo midbrain neurocytes, METHODS: The experiment was performed at the Cell Culture Room, Research Center, Medical College, Jiangsu University from March to April 2006. The rat embryonic midbrain neurocytes were cultured for 16 days in vitro. The effects of different contents of VC (5,10,25,50 μ mol/L) and VE(10,25,50,100μ mol/L), compound ( VC 25 μ mol/L plus VE 50μ mol/L and VC 50μ mol/L plus VE 100μmol/L) were observed. Cells were collected at day 10 after culture. Change in cell form, contents of protein and malonaldehyde (MDA) as well as the activity of superoxide dismutase (SOD) were analyzed with image. REULTS: (1)In the presence of VC, VE and compound VC with VE could promote midbrain neurocytes of rat embryonic proliferation, increase amount of cell concertration. (2)The amount of total protein of nerve cell obvious increased in the VC 10, 25 μmol/L group, VE 10-50μ mol/L group, VC 25 μmol/L+ VE 50 p, mol/L group relatively as compared with normal control group. (3)Compared with the normal control group, the content the of SOD increased and the content of MDA decreased in the VC10,25μmol/L group, VE 25,50 μmol/L group, VC 25 μmol/L+VE 50μmol/L group. (4)The amount of SOD in the VC50 μmol/L group, VE 100 μmol/L group and VC 50 μmol/L+VE 100μmol/L group were lower than that in the normal control group, while the content of MDA was higher than the normal control group. CONCLUSION: VC, VE and combination of VC and VE of a certain dosage can obviously improve antioxidation ability and promote the differentiation and proliferation of midbrain neurocyte in vitro,
出处 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2007年第3期447-450,共4页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 江苏省教育厅自然科学基金资助项目(04kjB310020)~~
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