摘要
从宜昌市夷陵区草坪型高羊茅（Festuca arundinacea．）分离出引起叶斑病的病原真菌，对该菌进行了形态学观察、致病性鉴定、核糖体DNA—ITS序列分析和生物学特性研究。结果表明：该病原菌能侵染高羊茅，在26℃和光照下，在PDA培养基上培养6d后菌落呈墨绿色，菌丝有隔，分生孢子有4个横隔膜，平均大小为30．6～17．0μm×10．9～13．6μm，中部3胞暗色，第3个细胞基部膨大；其核糖体DNA—ITS序列分析表明，该菌与GenBank中近缘弯孢（Curvularia affinis）的同源性是100％；结合形态学特征和致病性测定，认为该菌为C．affinis，这是C．affinis引起草坪型高羊茅叶斑病在我国的首次报道；生物学特性的研究表明，该菌菌丝生长的最适温度为25～30℃，最适pH范围为6～7，最适碳源为蔗糖，最适氮源为Ca（NO3）2；菌丝和分生孢子的致死温度分别为55℃ 10min和60℃10min。
A pathogen was isolated from Festuca arundinacea of a turf in Yiling district of Yichang, Hubei Province. The morphological characteristics, pathogenicity, the DNA sequence of ribosomal ITS and biological characteristics of the pathogen were studied. In a test of pathogenicity, the organism was able to infect F. arundinacea in vitro. After cultivation on PDA at 26℃ under continuous fluorescent light for 6 days, the colonies were black green and the hyphae were septate. Spore size was 30. 6～17. 0 μm × 10. 9～13.6 μm, mostly with four-septa. The central cell was dark and the third cell from the base was the largest. The DNA sequence of ribosomal ITS of the isolates was found to match 100% with an ITS sequence of Curvularia affinis in GenBank. This is the first report of leaf spot caused by C. affinis on F. arundinacea in China. The studies on biological characteristic revealed that the optimal temperature for the mycelial growth was 25～30℃, and the optimal pH for mycelial growth was pH 5～7. Among carbon and nitrogen sources, sucrose and calcium nitrate were best for mycelial growth. The lethal temperature for mycelial growth and spores gemination were respectively 55℃ and 60℃ for 10 min.
出处
《草地学报》
CAS
CSCD
2006年第4期315-318,327,共5页
Acta Agrestia Sinica
基金
国家外专局2003年度引智项目资助
关键词
高羊茅
近缘弯孢霉
叶斑病
病原鉴定
生物学特性
Festuca arundinacea
Curvularia affinis
Leaf spot
Pathogen identification
Biological characteristics
