摘要
目的通过重组cDNA表达文库的血清学分析(SEREX)技术获得系统性红斑狼疮(SLE)血管炎相关自身抗体所对应自身抗原的cDNA序列。方法收集SLE病人血清作为筛选血清,采用SEREX技术筛选人微血管内皮细胞(HM VEC)cDNA文库。结果通过两轮筛选获得11个阳性克隆。通过生物信息学分析发现这11个阳性克隆cDNA序列编码三种不同的自身抗原,分别为SSA、SSB和YB-1。其中YB-1尚未被报道过与SLE血管炎相关。结论首次发现YB-1与SLE血管炎相关。通过SEREX技术,我们可能找到有助于SLE诊断、治疗和判断预后的自身抗原。
Objective To screen autoantigens associated with vasculitis in systemic lupus erythematosus （SLE） by serological analysis of recombinant cDNA expression library （SEREX）. Methods A cDNA expression library derived from human microvascular endothelial cells （HMVEC） was screened with pooled sera from patients with SLE. Pooled sera were preadsorbed to reduce the background sera activity. The obtained preadsorbed pooled sera of patients were used for immunoscreening. Immunoscreening procedure was done according to the instruction manual provided by the manufacturer （Stratagene）. Results Eleven distinct positive clones were identified. Bioinformatic analysis revealed these 11 positive clones encoding three different proteins： SSA, SSB and YB-1. As for YB-1, there are hitherto no reports unveiling its association with vasculitis in SLE. Conclusion The association of YB-1 with vasculitis in SLE was found for the first time in this study. SEREX has offered a new strategy to explore the autoantigens related to SLE. By SEREX, we may find out the autoantigen which is helpful to the diagnosis and treatment of SLE, and to the prognostication concerned.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期132-134,149,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家863计划(资助号2002AA222031)资助
