摘要
目的观察纳洛酮对缺氧心肌组织再灌注血流及前列腺素代谢产物生成的影响。方法30只新西兰大白兔随机分为:再灌注组10只,结扎冠状动脉左前降支1 h再开放4.5 h;纳洛酮组10只,手术过程同前,同时静注纳洛酮;假手术组10只,手术过程同前,不结扎前降支动脉。用激光多谱勒血流测定仪测定再灌注组及纳洛酮组结扎前、后及再灌注4.5 h后缺血区心肌再灌注血流量;放射免疫法测定各组再灌注4.5 h后的血浆6-酮-前列腺素F1α、血栓素B2含量。结果(1)结扎前,再灌注组及纳洛酮组局部心肌组织血流无差别;再灌注后,两组血流均较前下降,再灌注组的缺血区血流量明显低于纳洛酮组(P<0.01)。(2)再灌注后,再灌注组、纳洛酮组血浆血栓素B2含量及血栓素B2/6-酮-前列腺素F1α比值显著高于假手术组,再灌注组又高于纳洛酮组,各组间血浆6-酮-前列腺素F1α含量无显著性差异。结论纳洛酮可以抑制缺血/再灌注后家兔心肌血栓素B2的生成,改善再灌注部位血流。
Objective To study the effect of Nalonxe on 6-Keto-PGF1α/TXB2 generated from ischemic and reperfused myocardium. Methods 30 mature male anesthetized New Zealand white rabbits were divided into three groups randomly： reperfusion group （coronary left anterior descending artery was occluded for 1 hour and reperfused for 4.5 hours）, Nalxone group （ ischemia/reperfusian combined with Naloxone intravenous injection） and sham-operation group. The blood flow in ischemic area was assayed with laser-doppler blood flow detector and TXB2/6-Keto-PGF1α, of serum were detected using radioimmunoassays. Results After reperfusion, 1. The reperfusion flow in ischemic area of reperfusion group was significantly lower than that of Nalxone group. 2. The concentration of TXB2and the ratio of TX2/6-Keto-PGF1α of reperfusion and Nalxone group were much higher than those of sham-operation group（P 〈 0.01 and 0.05, respectively）, while those of reperfusion group were obviously higher than those of Nalxone group. （P 〈 0.01）. Conclusion Ischemia/ reperfusion may lead to generation and discharging of TXB, from injured myocardium. Naloxane may significantly inhibit it and improve local non-reflow phenomena.
出处
《中国微循环》
北大核心
2006年第6期393-395,共3页
Journal of Chinese Microcirculation
