摘要
采用盆栽方法,研究了Cd污染对烤烟叶片光合特性、烟叶品质及其产量的影响。结果表明,随着Cd处理浓度的增加,烟叶净光合速率(Pn)和气孔导度(Gs)逐渐降低,胞间CO2浓度(Ci)随Cd浓度的增加逐渐增大;光系统Ⅱ活性(Fv/Fo)、最大光能转换效率(Fv/Fm)、光化学猝灭系数(qP)、非光化学猝灭系数(NPQ)、电子传递的量子产率(ФPSⅡ)和有效电子传递速率(ETR)则随Cd浓度的增加而降低;烤烟烟叶中的糖/碱比和氮/碱比升高,化学成分组成趋于不协调,不利于烟叶香吃味的形成。
In order to understand the mechanism of the effect of Cd pollution on photosynthesis, quality and yield of tobacco leaves, pot experiments were carried out at the Research Station of Henan Agricultural University during 2002 - 2004. Soil having a pH value of 7.5 and containing 8.53 g· kg^ - 1 organic matter, 0.89 g· kg^ - 1 total N, 20.42 mg· kg^ - 1 olsen-P, 238mg· kg^ - 1 NH4OAcK, and 0.11 mg· kg^ - 1 Cd was used in the experiments. 15 kg of sieved soil was placed in each 36 cm × 42 cm pot. Fertilizers used were （NH4）2SO4,KNO3 and KH2PO4 .Prior to sowing 3 g of N,4.5 g of P2O5 and 9 g of K2O were applied to each pot. The cultivar used in the experiments was Yunyan 85, with 20 leaves left after topping. The liquid Cd（Ac）2 was applied to each pot as sewage with concentrations of 3,6,10,30,60 and 100 mg· kg^- 1 after transplanting. Clean water was used as comparison. The experiment was arranged in a completely randomized design with three replications. The net photosynthetic rate （Pn）, intercellular CO2 concentration （Ci） and stomatal conductance （ Gs ） of three identical functional leaves from the same position were determined during rosette, budding and harvest stages using a portable photosynthetic system （Li-6400） . Maximal fluorescence （ Fm）, fixed fluorescence （ Fo ）and steady fluorescence （Fs） were determined after 30- minute dark adaptation while maximal fluorescence in the light （Fm＇） was measured after a certain length of light period （Hansatech FMS2 fluorometer）. The same portion of each leaf was used for both kinds of measurements. PS 1] activity（ Fv/Fo）, PS Ⅱ maximum light energy transformation （Fv/Fm）, chemical quenching coefficient（qP）, non-photochemical quenching coefficient （ NPQ）, the apparent photosynthetic electron transport rate （ETR） and the ratio of photochemical quantum yield of PS Ⅱ （φPS Ⅱ） were calculated according to the following formulas： Fv / Fo = （Fm - Fo ） / Fo , Fv / Fm = （Fm - Fo ） / Fm
出处
《生态学报》
CAS
CSCD
北大核心
2006年第12期4039-4044,共6页
Acta Ecologica Sinica
基金
国家烟草专卖局重大招标资助项目(110200201005)~~
关键词
CD污染
烤烟
光合特性
品质
产量
Cd pollution
tobacco
photosynthetic characteristics
quality
yield
