摘要
目的检测复方甘草酸苷注射液(商品名美能)治疗慢性特发性荨麻疹前后患者外周血CD3+T细胞细胞因子水平,探讨美能治疗慢性荨麻疹的可能免疫学机制。方法分离外周血淋巴细胞,刺激物刺激细胞,增加细胞内细胞因子的表达,然后应用流式细胞仪定量测定Th1/Th2细胞因子的水平,观察美能治疗慢性特发性荨麻疹前后Th1/Th2细胞因子水平的变化并作疗效评估,进一步作Th1/Th2细胞因子水平和疗效水平相关性分析。结果慢性特发性荨麻疹患者外周血Th1细胞因子明显低于正常人,而Th2细胞因子水平升高,美能治疗后,患者Th1细胞因子水平较治疗前升高,Th2细胞因子水平下降。Th1细胞因子水平和疗效水平呈正相关,Th2细胞因子水平和疗效水平呈负相关。结论美能通过逆转慢性荨麻疹患者失衡的Th1/Th2免疫反应是其达到临床疗效的可能机制之一。
Objective To investigate cytokine profiles in peripheral blood and to explore immune effects of Glycyrrhizin on patients with chronic idiopathic urticaria （CIU）. Methods 20 cases and 20 healthy controls were involved in this study. The peripheral blood lymphoeytes were isolated and stimulated with PMA, then intraeellular cytokines were measured through flow eytometry analysis, finally profiles of eytokine were compared with administration and glycyrrhizin. The curative effect such as improvement of itch, wheal, and so on were assessed too. Results The levels of IFN-γ and IL-2 were decreased in CIU in comparison with healthy controls. After treatment of glycyrrhizin, the levels of INF-γ and IL-2 were augmented, while the level of IL-4 were not obviously decreased. The levels of IFN-γ, IL-2 positively correlated with all indexes of cure, the levels of ID-4 negatively did. Conclusion There is Th1/Th2 imbalance in CIU with a dechne to Thl type response, Glycyrrhizin can reverse the imbalance to reach the clinic cure.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2006年第4期214-216,共3页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
