摘要
用扫描电子显微镜和光学显微镜的染色观察，研究了棉铃虫ＨｅｌｉｃｏｖｅｒｐａａｒｍｉｇｅｒａＨｕｂｎｅｒ３龄幼虫腹部神经－肌肉的分布，表明其腹纵肌细胞适合于作电生理细胞内记录。用电生理细胞内微电极记录法研究了棉铃虫三个品系：对照品系、功夫菊酯抗性品系（Ｃｙ－Ｒ，抗性指数１３．４）和氰戊菊酯抗性品系（Ｆｎ—Ｒ，抗性指数３７．９）的３龄幼虫神经－肌肉材料对相应杀虫剂的神经敏感性。结果表明：用１０－６ｍｏｌ／Ｌ的功夫菊酯处理Ｃｙ—Ｒ，在３０ｍｉｎ内有４０％的个体对药剂无反应，另外６０％的个体产生反应所需的时间是对照品系的３．５倍。用１０－６ｍｏｌ／Ｌ的氰戊菊酯处理Ｆｎ—Ｒ，在３０ｍｉｎ内有４７％的个体对药剂无反应，另外５３％的个体产生反应所需的时间是对照品系的４．２倍。这些结果说明神经敏感性降低是棉铃虫对拟除虫菊酯抗药性的重要机制。
The morphology of the 3rd instar larval cotton bollworm (Helicoverpa armigera Hubner) ventral nerve and muscle was studied by scanning electron and light microscopes. The results of observation showed that all of the ventral longitudinal muscle cells are suitable for the electrophysiological intracellular recording. The nerve sensitivity to the corresponding pesticides of 3rd instar larval neuromuscular synapses of three strains of the cotton bollworm including the control, the cyhalothrin resistant (Cy- R, resistance ratio: 13.4) and the fenvalerate resistant (Fn-R, resistance ratio: 37.9)strains was studied by using the electrophysiological intracellular microelectrode recording technique.The results showed that when Cy-R was treated by 10-6mol/L cyhalothrin. 60% individuals had the repetitive discharge within 30min; however, the reaction time was 3.5 times as much as that of the control. And the other 40%individuals had no reaction. When Fn-R was treated by 10-6mol /L fenvalerate,53% individuals had the repetitive burst discharge within 30min, however, the reaction time was 4.2 times as much as that of the control' And the other 47% individuals had no reaction. It implies that the decreased nerve sensitivity is an important pyrethroid resistance mechanism of cotton bollworm.
出处
《昆虫学报》
CAS
CSCD
北大核心
1996年第4期347-353,共7页
Acta Entomologica Sinica
关键词
棉铃虫
拟除虫菊酯
抗药性
神经敏感性
Helicoverpa armigera, pyrethroid, resistance, nerve sensitivity
