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高效液相色谱法测定银杏叶提取物中黄酮甙含量 被引量:6

DETERMINATION OF FLAVONOIDS IN EXTRACT OF GINKGO BILOBA LEAVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
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摘要 用高效液相色谱法测定银杏叶提取物经酸水解后黄酮甙元含量.采用C18柱,甲醇:水:磷酸(55:44.5:0.5)为流动相,检测波长370nm,方法回收率95.1%~103.2%,变异系数(CV)3.17%,10批样品含测平均为25.4%. A HPLC method has been developed for the determination of Flavonoids in extract of Ginkgo biloba, leaves. (EGb). Procedure includes hydrolpsis of flavonoids and subsequent quantitative chromatographic assay of the obtained aglycones on a C18 colunm using methanol, water and phosphoric acid (55: 44.5: 0.5) as mobile phase, and UV detection at 370 mm. The results have shown that the recoveries were between 95.1%-103.2% and CV was 3.17%. The average content of ginkgo flavanol glycosides in EGb was 25.4%.
作者 李典鹏 方宏
机构地区 广西植物研究所
出处 《广西植物》 CAS CSCD 北大核心 1996年第3期292-294,共3页 Guihaia
关键词 黄酮甙 银杏叶提取物 高效液相色谱 HPLC flavanol glycosides quercetin extract of Ginkgo biloba
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