摘要
创面愈合是一个复杂的过程。文献中关于创面愈合过程中，上皮细胞生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）基因表达变化的研究较少，为此设计了本项研究。采用Ｗｉｓｔａｒ大鼠，在脊柱两侧制作１．５ｃｍ×１．５ｃｍ中厚断层皮肤缺损创面４个，于伤后第４，８，１２和１６天取材，用地高辛标记ＥＧＦｃＤＮＡ探针原位杂交方法，观察创面愈合过程中，ＥＧＦ基因活化表达的ｍＲＮＡ在组织中的分布变化。发现：在伤口愈合的全过程中，ＥＧＦ基因均有明显表达，以伤后第８天表达最强。提示：应用某种方法促进ＥＧＦ基因表达。
In order to understand the expression change of epidermal growth factor (EGF) gene and its distribution in tissue during wound healing, 12 Winstar rats were divided into 4 groups. In each side of the back of every rat, 4 wounds of 1.5cm×1.5cm in size were made. After 4,8,12,16 days, one group of rats was sacrificed and tissues from wound were collected. By DIG labelled probe hybridization in situ technique, EGF gene mRNA was detected. It was shown that EGF gene expression was evident during the whole stage of wound healing, and the peak was in 8th day. It suggested that the promotion of EGF gene expression may lead to wound healing earlier.
出处
《中国修复重建外科杂志》
CAS
CSCD
1996年第3期133-135,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金
