摘要
应用荧光光谱法和紫外吸收光谱法研究了在生理条件下(pH=7.4)磺胺二甲氧嘧啶(SDM)与牛血清白蛋白(BSA)的相互作用模式.研究表明,磺胺二甲氧嘧啶对BSA内源性荧光的猝灭属于形成了复合物的单一静态猝灭过程,其猝灭速率常数为7.35×1012L.mol-1.S-1,结合常数为1.04×105L.mol-1,结合位点数为1.03.并考察了金属离子Fe3+、Mn2+、Ca2+、Zn2+、Cu2+存在下,对磺胺二甲氧嘧啶与BSA结合常数的影响.
The interaction between sulfadimethoxine （SDM） and bovine serum albumin （BSA） in physiological condition was studied by fluorescence and spectrophotometry. The experiment demonstrated that the combination for sulfadimethoxine with BSA was a single static quenching process. The quenching rate constant is 7.35 × 10n L · mol^-1 · S^-1, the binding constant is 1.04 × 10^5 L · mol^-1, and the number of binding sites is 1.03. Effect of Fe^3＋ , Mn^2＋ , Ca^2＋ , Zn^2＋ and Cu^2＋ on the binding constant of sulfadimethoxine to BSA was also studied.
出处
《南昌大学学报（工科版）》
CAS
2006年第4期316-318,335,共4页
Journal of Nanchang University(Engineering & Technology)
基金
长江学者和创新团队发展计划资助项目(IRT0540)
江西省教育厅基金资助项目(赣教技字[2005]-71号)
