摘要
目的探讨结核分枝杆菌耐药性与whiB7基因表达水平之间的关系。方法以耐受利福平、异烟肼、链霉素、乙胺丁醇和氟喹诺酮的结核分枝杆菌临床分离株为研究对象。利用实时定量PCR的方法.检测药物刺激结核分枝杆菌后whiB7基因表达水平的变化。结果除利福平刺激相应的菌株后。whiB7基因的表达水平变化不明显外.其余药物的刺激均会引起结核分枝杆菌耐约株的whiB7基因表达水平发生显著变化。结论结核分枝杆菌的whiB7基因是对多种抗生素刺激发生反应的基因。推测whiB7的表达参与结核分枝杆菌的耐药过程。
Objective To investigate the relationship between the expression of whiB7 gene and the muhidrug resistance of the Mycobacterium tuberculosis(MTB). Methods The antibiotics, including rifampin, isoniazid, streptomycin, ethambutol and fluoroquinolones, were used to stimulate the clinic isolates of MTB respectively. After having been induced expression of whiB7 MTB was measured by quantitive real time PCR. Results The expressing level of whiB7 gene was significantly improved after the MTB stimulated by isoniazid, streptomycin, ethambutol and fluoroquinolones except for the rifampin, Conclusion WhiB7 is widely involved with the resistance of MTB to multiple antibiotics.
出处
《结核病与胸部肿瘤》
2006年第4期243-247,共5页
Tuberculosis and Thoracic Tumor
基金
该项目获北京市科委2006年度国际合作项目资助