摘要
目的 明确空心莲子草类盐抗汉坦病毒（HV）的作用机制。方法 采用噻唑兰（MTT）比色法检测细胞活性、免疫荧光法（IFA）和逆转录聚合酶链反应（RT-PCR）检测病毒滴度和病毒核酸，以确定空心莲子草类盐细胞内抗HV作用。结果 空心莲子草类盐对HV无直接灭活作用，也不能阻止HV的吸附，这表现在各药物浓度组病毒滴度均在5．7以上；空心莲子草类盐有抑制HV生物合成作用，在质量浓度为40-320mg·L^-1内，随着药物质量浓度的增加，病毒滴度、细胞培养液中病毒核酸的阳性稀释度逐渐降低，抗病毒指数逐渐增加，并表现出量效关系，药物治疗组的病毒滴度明显低于病毒对照组（P〈0.05）。结论 空心莲子草类盐在体外通过抑制病毒生物合成而发挥抗HV作用。
OBJECTIVE To investigate anti-hantavirus （HV） activity of compounds obtained from griseb extracts in vitro. METHODS The arlti-HV activities were investigated. Cell activity was determined by MTT colorimetric assay, Viral liters were measured by immunofluorscence assay （IFA） and HV-RNA was detected by reverse transcription polymerase chain reaction （RT-PCR）.RESULTS No antiviral abilities of directly-killing and adsorption-bilking were obtained while viral titers were over 5,7 at all concentrations, Anti-biological synthesis was observed at the doses ranging from 40 to 320 mg·L^-1, both viral titers and titers of HV-RNA in culture media decreased correspondingly, whereas the antiviral index increased. The viral titers in the tratment group were significantly lower than that of HV control （ P 〈 0.05）. CONCLUSION Griseb may inhibit HV biological synthesis instead of directly inactivating these viruses or blocking their adsorption to susceptible cells in vitro.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第22期1713-1715,共3页
Chinese Pharmaceutical Journal
基金
湖北省卫生厅资助项目(WJ01550)
