摘要
目的探讨北京地区汉族人群中血小板活化因子乙酰水解酶(platelet-activating factor-acetylhydrolase,PAF-AH)基因V279F变异与冠心病的关系。方法对124例冠心病患者和103例对照者进行病例对照研究。采用等位基因特异性聚合酶链式反应(AS-PCR)的方法确定PAF-AH的基因型。结果在北京地区汉族人群PAF-AH的3种基因型中,VV型基因型的发生频率(92．2%)最高,VF型(5．8%)次之,FF型(2．0%)最低。冠心病组中V279F变异携带者的频率(19．3%)显著高于对照组(7．8%,P＜0．05)；F等位基因的频率(12．1%)也显著高于对照组(4．9%,P＜0．01)。冠心病组中心肌梗死组V279F变异携带者的频率和F等位基因的频率均显著高于非心肌梗死组(27．3%对13．0%,17．3%对8．0%,均为P＜0．05)。多因素logistic回归分析显示冠心病患者V279F变异的OR值为1．919(95% CI：1．448～2．544,P=0．033)。结论PAF-AH基因V279F变异可能是冠心病的一种遗传危险标记。
Objective To explore the relationship between genetic variation in PAF-AH V279F and coronary heart disease among Han population in Beijing. Methods A case-control study was held which enrolled 124 patients with coronary heart disease and 103 normal subjects. The genotype of PAF-AH V279F was determined with allele-specific polymerase chain reaction （AS-PCR） method. Results The highest frequency of PAF-AH V279F genetic variation was VV genotype（92.2%）, the next was VF genotype （5.8%） and the lowest was FF genotype （2. 0%） among the studied Han population in Beijing. In the coronary heart disease group the frequency of 279 V→F carriers was significantly higher than in the control group （19.3% vs. 7.8%, P〈0.05） and F allele frequency was also higher （12.1% vs. 4. 9%, P〈0.01）. Among the coronary heart disease group, the V279F variation frequency and the F allele frequency were significantly higher in patients with myocardial infarction than in those without myocardial infarction （27.3% vs. 13. 0%, 17.3% vs. 8. 0%, both P〈0.05）. In multiple logistic regression analysis, the odds ratio（OR） of V279F genetic variation for coronary heart disease was 1. 919 （95% CI：I. 448-2. 544,P= 0. 033）. Conclusions The PAF-AH V279F genetic variation may be a novel genetic marker for high risk of coronary heart disease.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2006年第12期895-899,共5页
Chinese Journal of Geriatrics
基金
中央保健委员会保健科研基金资助项目(004)
关键词
冠状动脉疾病
动脉硬化
心肌梗死
血小板活化因子水解酶
Coronary disease
Arteriosclerosis
Myocardial infarction
Platelet-activating factor hydrolase
