摘要
目的研究黄芪多糖(astragalus polysaccharide,APS)对大鼠脑出血(ICH)后血肿周围核因子-κB (nuclear factor-kappa B)蛋白表达的影响,探讨黄芪多糖抗炎的脑保护机制。方法用Ⅶ型胶原酶注射到大鼠右侧苍白球诱导脑出血模型,并用APS干预。用免疫组化方法检测血肿周围组织NF-κB表达的动态变化,透射电镜观察术后3 d血肿周围神经元超微结构的变化及神经行为学评分。结果与模型组比较,治疗组干预后3 d和5 d大鼠神经行为学评分明显减少(P<0．05),6 h、1 d、3 d、5 d时NF-κB阳性细胞数明显减少(6 h、1 d P<0．05,3、5 d P<0．01)。神经元超微结构改变亦比模型组减轻。结论黄芪多糖可抑制NF-κB激活,减轻炎症反应,改善神经功能缺损症状。
Objective To investigate the expression of nuclear factor-kB（NF-kB） around the hematoma by the intervension of APS in rat,to study mechanisms of APS protecting brain and its effect on inflammation after intracerebral hemorrhage. Methods ICH models was induced in rats by injection of Ⅶ type collagenase into the right globas pallidus. APS was used as the intervension drug. Immunohistochemisty was used to investigate the dynamic changes of the expression of NF-kB in the tissue surrounding hematoma in ICH rats. Ultrastructures of the cells around the hematoma were observed with transmission electron microscope. Results Compared with model group, the scores of neurological behavior in treatment group rats were decreased significantly at 3 day and 5 day（P 0.05）, NF-kB positive cells were decreased significantly at 6h, 1 day, 3 day and 5 day（6h, ld P 〈 0.05,3d,Sd P〈 0.01 ）. The pathological changes of the treated group were milder than the model group on 3d postoperative. Conclusion APS can inhibit the activation of NF-kB and effectively alleviate inflammatory response. It can accelerate the recover of neurological function deficit after ICH.
出处
《中国基层医药》
CAS
2006年第11期1766-1768,I0001,共4页
Chinese Journal of Primary Medicine and Pharmacy
