摘要
利用随机引物多聚酶链式反应(AP-PCR)技术对来自江苏省连云港海区5种重要的水产贝类(文蛤、青蛤、杂色蛤仔、四角蛤蜊和缢蛏)的遗传多样性进行了分析。由选取的28条随机引物共扩增出482个DNA片段,遗传多态性高达100%,而帘蛤科内的3种贝类的遗传多态性平均为91%。利用NTSYSpc生物学软件计算出5种贝类种间的遗传相似系数和相对遗传距离,聚类分析(UPGAM)结果表明:5种贝类在系统进化上的关系较远,它们可聚类为3组,它们的平均遗传相似系数为0.520,其中Ⅰ组有文蛤和青蛤,组内遗传相似系数为0.570;Ⅱ组有四角蛤蜊,它与Ⅰ组的遗传相似系数为0.561;Ⅲ组有杂色蛤仔和缢蛏,组内的遗传相似系数为0.544。
The genetic diversity of five important mollusks along the coast of Lianyungang in Jiangsu province was analyzed by the method of arbitrarily primed-PCR. 482 DNA bands were amplified with 28 random primers and there were all polymorphic DNAs, while the polymorphic rate was about 95 per centage among the three species in Veneridae. The clustering analysis of 5 species showed that they could be subdivided into 3 groups, among which the average coefficient was about 0. 520. Results showed that Meretrix meretrix and Cyclina sinensis were in the same group, and their coefficient was about 0. 570, Ruditapes variegate and Sinonovacula constricta could be clustered as the second group, and their coefficient was about 0. 544, while Mactra veneriformis was in the third group.
出处
《药物生物技术》
CAS
CSCD
2006年第6期418-421,共4页
Pharmaceutical Biotechnology
