摘要
通过SOE法(重叠延伸,Genesplicingbyoverlapextension),扩增cfp10-esat6融合基因,与穿梭整合质粒pMV361构建成重组质粒。另将BCGα抗原(α-Ag)信号肽序列与上述重组质粒构建成另一重组质粒。将两种重组质粒分别导入BCG菌构建融合型重组卡介苗和分泌型重组卡介苗,热诱导后分析其表达产物。本研究成功构建并表达了CFP10-ESAT6融合及分泌表达的重组BCG,为发展新型结核病疫苗打下了一定的基础。
This study was conducted to amplify the cfp10-esat6 fusion gene by SOE and insert into the integrating shuttle plasmid pMV361 to form the recombinant plasmid. Then another recombinant plasmid was constructed by insertingα-A g signal sequence of BCG. The two recombinant plasmids were introduced into BCG and the induced products from recombinant BCG were analyzed. In conclusion,the successful construction of rBCG expressing the fusion protein CFP10-ESAT6 will be the base of the development of novel Mycobacterium tuberclosis vaccines.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2006年第6期1298-1302,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30271172)
