摘要
目的研究脉络膜黑色素瘤中PTEN蛋白表达及其与脉络膜黑色素瘤局部侵袭转移机制的相关性。设计回顾性病例系列。研究对象36例脉络膜黑色素瘤和2例正常葡萄膜组织。方法对36例脉络膜黑色素瘤进行组织病理学分析,应用免疫组化方法检测肿瘤组织及正常葡萄膜组织中PTEN的表达,并分析其与组织病理学特征以及同肿瘤侵犯巩膜导管与否之间的关系。主要指标病理组织学、免疫组织化学结果。结果36例脉络膜黑色素瘤组织中,肿瘤侵犯巩膜导管15例(41．7％), PTEN阳性表达20例(55．6％);2例正常葡萄膜组织全部阳性。巩膜导管受侵犯组和未受侵犯组阳性表达率分别为33．3％(5/15例)和76．2％(16／21例)(X2=5．143,P=0．026)。结论PTEN蛋白在脉络膜黑色素瘤侵犯巩膜导管时明显降低。推测PTEN基因的正常表达在抑制脉络膜黑色素瘤细胞的聚集黏附过程中具有重要作用。
Objective To investigate the expression of tumor suppressor gene PTEN in malignant melanoma of choroid and to analyze the correlation between PTEN and mechanism of invasion and metastasis of the tumor. Design Retrospective case series. Participants Thirty-six cases of malignant melanoma of choroid and two cases of normal choroid tissue. Main Outcome Measures Pathological and immunohistochemical staining （PowerVision^TM） results. Methods PTEN expression was compared with the clinical pathologic features and the occurrence of tumor invasion into the scleral canal. Results The positive expression rate of PTEN in melanoma was 55.6% （20/36）, while expressions in two cases of normal cboroid tissue were both positive. Positive expression rate of PTEN in scleral canal invasion group （33.3%, 5/15 ） was significantly lower than that of the opposite group （76.2%, 16/21 ） （χ^2=5.143, P=0.026）. Conclusions PTEN expression in choroidal malignant melanoma was lower than that in normal choroid tissue and was significant lower in group of tumor extended into scleral canal. Normal expression of PTEN may play an important role in suppressing focal adhesion formation, spreading and migration of melanoma cells.
出处
《眼科》
CAS
2006年第6期381-384,共4页
Ophthalmology in China
