摘要
目的探讨高肺血流对大鼠肺动脉胶原水平及代谢的影响。方法雄性SD大鼠32只,随机均分为分流组和对照组,对分流组大鼠行腹主动脉-下腔静脉穿刺术建立高肺血流动物模型。分别在分流术后4、11周2个时间点处死大鼠留取肺组织标本(每组n=8)。制作肺组织石蜡切片,以免疫组织化学方法检测肺动脉胶原Ⅰ、胶原Ⅲ、金属基质蛋白酶-13(MMP-13)及其抑制物-1(TIMP-1)表达的变化。结果分流后4、11周,与对照组比较,大鼠肺动脉胶原Ⅰ、胶原Ⅲ、MMP-13及TIMP-1表达明显增强(P<0.05)。结论高肺血流上调大鼠肺动脉胶原Ⅰ和胶原Ⅲ表达,该作用可能参与介导高肺血流致肺血管结构重建的机制。
Objective To explore impact of high pulmonary blood flow on the content and metaholism of collagen in rats. Methods Thirty - two male SD rats were randomly divided into shunt group and control group. Rats in shunt group were subjected to an abdominal aorta - inferior vena eava shunt to create an animal model of high pulmonary blood flow. In control group, rats experienced the same experimental processes except the shunting procedure. After 4 and 11 weeks of experiment, these changes of pulmonaryartery collagen Ⅰ, col- lagen Ⅲ,matrix metalloproteinase(MMP- 13)and tissue inhibitor of metalloproteinase(TIMP- 1) protein expression of rat Were investigated by immunohistochemistry. Results After 4 weeks and 11 weeks of shunt,the collagen Ⅰ,collagen Ⅲ ,MMP- 13 and TIMP- 1 of pulmonary artery in rats of shunt group increased significantly compared with those of control group, respectively(all P 〈0,05). Conclusions High pulmonary blood flow up - regulates the pulmonary artery collagen Ⅰ and collagen Ⅲ protein expression in rats,which may be involved in the mechanism of pulmonary vascular structural remodeling induced by high pulmonary blood flow.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第23期1635-1636,共2页
Journal of Applied Clinical Pediatrics
基金
国家杰出青年科学基金项目资助(30425010)
国家重点基础研究发展规划(2006CB503807)
北京市自然科学基金项目资助(7052043)
关键词
高肺血流
胶原
大鼠
high pulmonary blood flow
collagen
rat