摘要
目的 研究胎鼠胃上皮细胞增殖、凋亡的时空规律及其与胃形态发生的关系。方法 每间隔0．5d取E11～15．5d小鼠整胎，横断连续切片，观察胃形态发生，体视学法测量胃贲门、幽门、大弯、小弯上皮分裂细胞密度（DMC）、凋亡小体密度（DAB）。结果①E11．5d胃开始发育，E12d胃底膨出，胃大、小弯出现。②上皮细胞DMC峰值均在DAB峰值之前。③贲门处DMC无明显峰值，DAB峰值在E11．5、12．5、13．5d；幽门处DMC峰值在E12．5d，DAB峰值在E14d；大弯DMC峰值在E11．5、12d时，DAB值持续较低；小弯DMC峰值在E11．5d，DAB峰值在E12．5d。结论 胎鼠胃上皮细胞的增殖及凋亡与胃发生有密切的时空关系。
Objective To study the spatiotemporal distributions of epithelial proliferation and apoptosis, and the correlation of morphogenesis with them in developing stomach. Methods Serial sections of fetal mice aged 11 - 15.5 days were made. The morphogenesis of stomach was observed under light microscope. The density of mitotic ceils （DMC） and apoptotie bodies （DAB） of epithelium in the developing cardia, pylorus, greater curvature, lesser curvature were measured with stereological method. Results The morphological development of stomach took place on the 11^ th day of mouse embryo development. The fundus, greater curvature and lesser curvature were observed on the 12^th day. The peak value of DMC all appeared before the peak value of DAB. In the epithelium of cardia, no obvious peak value of DMC was observed and the peak value of DAB was on the 11.5^th, 12.5^th, 13.5^th day. In the epithelium of pylorus, the peak value of DMC was on the 12.5^th day, the peak value of DAB was on the 14^th day. In the epithelium of greater curvature, the peak value of DMC was on the 11.5^th and 12^th day, the DAB value was low. In the epithelium of lesser curvature, the peak value of DMC was on the 11.5^th day, the peak value of DAB was on the 12.5^th day. Conclusion There was very close spatiotemporal relationship between the cell proliferation, cell apoptosis and the morphological development of stomach.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期53-55,共3页
Journal of Third Military Medical University
基金
重庆市教委课题(渝教科9916)~~
关键词
胎鼠
胃
增殖
凋亡
形态发生
fetal mouse
stomach
proliferation
apoptosis
morphogenesis
