摘要
目的探讨复方双黄溶液对重度烧伤后大鼠肠黏膜的保护作用及细菌移位的抑制作用。方法将32只健康雄性SD大鼠随机分为正常对照组(不烧伤、自由进食水)、烧伤对照组(烧伤前1h胃饲1ml0·9%氯化钠溶液,烧伤后6h第二次胃饲,4次/d)、烧伤大黄组(烧伤前、后相同时间胃饲大黄溶液)和烧伤双黄组(烧伤前、后相同时间胃饲等量复方双黄溶液)。烧伤72h后计算大鼠胸腺指数、脾脏指数,检测肠黏液中SIgA含量,用放射免疫法计算细菌移位率,再通过透射电镜、扫描电镜观察肠黏膜病理形态变化。结果3个烧伤组大鼠的胸腺指数和脾脏指数与正常对照组相比,差别有显著性意义(P<0·05),烧伤双黄组大鼠脾脏指数和胸腺指数与烧伤对照组、烧伤大黄组相比,差别有显著性意义(P<0·05);4组大鼠脏器细菌移位率间差别有显著性意义(P<0·01),烧伤双黄组大鼠脏器细菌移位率与正常对照组、烧伤对照组和烧伤大黄组相比,差别均有显著性意义(P<0·05);3个烧伤组大鼠肠黏膜SIgA含量与正常对照组相比,差别有显著性意义(P<0·05),烧伤双黄组大鼠肠黏膜SIgA含量与烧伤大黄组、烧伤对照组相比,差别有显著性意义(P<0·05);烧伤双黄组大鼠肠黏膜病理形态改变明显减轻。结论复方双黄溶液可有效防治严重烧伤后肠黏膜损伤,抑制严重烧伤后肠道细菌移位。
Objective To discuss the mechanism of compound shuanghuang liquor on protecting the intestinal mucosa injury and inhibiting the bacterial translocation in rats with severe burn. Methods 32 healthy male SD rats were divided into four groups randomly. Rats in normal control group ate food and water freely without scald ; rats in scald control group were orally administered 1 ml 0. 9% saline an hour before they were scalded and six hours after they were scalded, four times per day; rats in scalded with rhubarb liquor treatment group and scalded with compound shuanghuang liquor treatment group were orally administered equal amount of rhubarb liquor and compound shuanghuang liquor respectively at the same time. 72 hours after the scald, the thymus index, spleen index, SlgA lever in intestinal mucus and the frequency of the bacterial translocation were calculated and detected; the changes in histopathology of intestinal mucosa were observed by light microscope and transmission electron microscope. Results Thymus index and spleen index in rats in three scald groups showed significance difference compared with normal control group ( P 〈 0. 05) ; there were no significant differences in thymus index and spleen index between compound shuanghuang liquor treatment group and scald control group or rhubarb liquor treatment group ( P 〈 0. 05 ). There was significant difference in the frequency of bacterial translocation in four groups ( P 〈0. 001 ), and the frequency of bacterial translocation in compound shuanghuang liquor treatment group differed significantly from that in other three groups ( P 〈 0. 05) ; Compared with the normal control group, the SlgA level in intestinal mucosa showed significant difference in three scald groups ( P 〈 0. 05 ). The IgA level in compound shuanghuang liquor treatment group differed significantly from rhubarb liquor treatment group and scald control group ( P 〈 0.05). The histopathology change of intestinal mucosa in compound shuanghuang liquor treatment gro
出处
《中国全科医学》
CAS
CSCD
2006年第24期2027-2030,共4页
Chinese General Practice
基金
河北省医学科学研究重点课题计划(05030)
关键词
烧伤
肠黏膜
细茼移位
大黄
复方双黄溶液
Burn
Intestinal mucosa
Bacterial translocation
Rhubarb
Compound shuanghuang llquor