摘要
目的:建立鉴别木香、川木香、土木香的方法。方法:Kromasil C_(18)柱(250 mm×4.6 mm,5 μm),甲醇-水梯度洗脱,检测波长为254 nm,流速为1 mL·min^(-1),柱温为30℃。结果:在选定色谱条件下,建立了稳定可控的高效液相色谱图;应用主成分分析法、系统聚类分析法将药材分成三类,并计算相似度。结论:应用高效液相色谱法鉴别木香、川木香、土木香是可行的。
Objective： To identify Radix Aucklandiae, Radix Vladimiriae and Radix Inulae. Method：The fingerprints were obtained from Waters 2695 instrument ,the HPLC was performed on a Kromasil C18 （250 mm ×4.6 mm, 5 μm） analytical column gradient eluted with methanol - water at the flow rate of 1 mL · min^ - 1. The temperature of column was 30 ℃. The UV detection wavelength was 254 nm. Result：Principal components analysis and clustering analysis were used in the identification of the samples, and the similitude was computed by computer. Conclusion： The method can identify Radix Aucklandiae, Radix Vladimiriae and Radix Inulae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第11期1574-1577,共4页
Chinese Journal of Pharmaceutical Analysis
基金
四川省科技攻关项目(No.2004)
关键词
高效液相色谱指纹图谱法
木香
川木香
土木香
系统聚类法
主成分分析
HPLC fingerprint
Radix Aucklandiae
Radix Vladimiriae
Radix inulae
clustering analysis
principal components analysis
