摘要
目的:建立高效毛细管电泳法同时分离测定红毛七药材中红毛新碱和塔斯品碱的含量。方法:采用 HP^(3D)CE G1600AX高效毛细管电泳仪,未涂渍熔融石英毛细管(75μm×50 cm,有效长度41.5 cm),压力进样50 mbar×5 sec,运行缓冲液为90mmol·L^(-1)的醋酸钠—15%甲醇溶液,冰醋酸调节 pH 至5.0,检测波长:245 nm,运行电压23 kV,温度30℃。结果:红毛新碱在21.2～106.0μg·mL^(-1)范围内线性关系良好(r=0.9987),塔斯品碱在33.6～168.0μg·mL^(-1)范围内线性关系良好(r=0.9981);平均加样回收率(n=6):红毛新碱99.46%,塔斯品碱100.13%。结论:该方法简单、快速、准确,结果可靠,重现性好,可用于红毛七药材中红毛新碱和塔斯品碱的含量测定。
Objective ：To determine of the content of Hongmaoxinjian and Taspine in Radix Caulophyllis by high performance capillary electrophoresis. Method： Untreated fused - silica capillary （50 cm × 75 μm, effective length is 41.5 cm）was used. Pressure injection of 50 mbar ×5 sec was employed,and the temperature was 30 ℃. The detection wavelength was set at 245 nm. The samples were analyzed with 15% methanol （including 90 mmol·mL^-1 sodium acetate） running buffer at 23 kV voltage. Results： The calibration curve of Hongmaoxinjian and taspine showed good linearity over the range of 21.2 - 106.0 μg·mL^-1（ r = 0. 9987 ） , 33, 6 - 168.0 μg·mL^-1 （ r = 0. 9981 ）,respectively. The average recoveries of the method were 99.46%, 100. 13% , respectively. Conclusion： The method is simple, rapid, accurate, reproducible and can be applied in the determination of Hongmaoxinjian and taspine in Radix Caulophyllis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第12期1706-1708,共3页
Chinese Journal of Pharmaceutical Analysis
基金
西安市重大科技创新项目(No:ZX05010)
关键词
高效毛细管电泳
红毛七
红毛新碱
塔斯品碱
含量测定
high performance capillary electrophoresis
Radix mination Caulophyllis
Hongmaoxinjian
Taspine
content determination
