摘要
性别控制是人类很早就关心的研究领域．因为许多重要的经济性状与性别有直接关系。在家畜生产中，性别控制应用潜力很大．可以大幅度提高生产力，节约生产成本。性别控制可使肉用家畜多产雄性后代，乳用家畜多产雌性后代。为了达到控制性别的目的，前人已经尝试了多种分离X、Y精子的方法，包括流式细胞分离仪法、免疫学法分离精子、干涉显微镜下分离X、Y精子、人血清白蛋白柱法和free-flow electrophoresis。然而，由于这些方法的可操作性、实用性以及成本等方面存在的问题而未能在实践中推广应用。
The effects of reproductive hormone on mouse embryo sexes were studied with in vitro fertilization（IVF）,in vitro cuhure（IVC） and sex identification. Results showed that it had no adverse effects on embryos cleavage and development after the embryos were incubated with the fertilization media with 17β-E2, FSH and progesterone respectively at the concentration of this study. Mice embryos sex ratios significantly deviated from 1：1（P〈0.01） and skewed in favor of males at the concentration of 17β-E2 attained 70 pg/mL and progesterone attained 99 pg/mL in IVF culture media respectively. 0.01-0.04 IU/mL FSH had no effects on the mouse embryo sexes.
出处
《分子细胞生物学报》
CSCD
北大核心
2006年第6期573-576,577,共5页
Journal of Molecular Cell Biology
基金
中国农科院人才课题。
关键词
小鼠胚胎
生殖激素
体外受精
性别鉴定
Mouse embryos. Reproductive hormone. In vitro fertilization. Sex identification
