摘要
应用蒙古沙土鼠制作的急性脑缺血动物模型，在５分钟和１０分钟脑缺血后，再灌注４小时～１４天，用免疫组化和电镜技术观察了不同实验组动物垂体前叶内两种促性腺激素阳性细胞的密度、染色反应和其超微结构的动态变化。结果表明：５分钟急性脑缺血及再灌注过程中出现的细胞密度、染色强度和超微结构的改变是暂时的、可逆的，在缺血后１周～２周内可基本恢复正常。但当脑缺血１０分钟再灌注后，垂体前叶促性腺激素细胞呈现较严重的缺血性损伤，部分细胞发生不可逆的退变反应。脑缺血后垂体前叶的促性腺激素细胞的病理损害是血清中促性腺激素含量变化的形态学基础。
Mongolian gerbils were used as transient cerebral ischemia animal models. After five and ten minutes of cerebral ischemia and at various recirculation times ranging from 4 hours to 14 days, changes of density, staining reaction and ultrastructure of gonadotropic cells in gerbil pituitary gland were observed. The results showed that changes of density, staining activities and ultrastructure during recirculation after five minutes of cerebral ischemia were temporary and reversible. The changes may disappear within 1～2 weeks after ischemia. After ten minutes of cerebral ischemia and recirculation, gonadotropic cells were seriously damaged, and histopathologic changes developed in some cells. After ischemia, pathological damages of gonadotropic cells in pituitary gland were morphologic basis of hormonal changes in blood.
出处
《泰山医学院学报》
CAS
1996年第3期173-178,共6页
Journal of Taishan Medical College
关键词
脑缺血
促性腺激素细胞
免疫组织化学
超微结构
Cerebral ischemia
Pituitary gland
gonadotropic cells
immunohistochemistry
ultrastructure
