摘要
目的观察类风湿关节炎(RA)患者血清中Ⅱ型胶原(CⅡ)抗体片段随病情发展的变化情况。方法采用酶联免疫吸附试验(ELISA)选取抗CⅡ抗体阳性患者,用免疫印迹方法检测其CⅡ抗体片段阳性情况。结果112例RA患者及120名健康对照人群中CⅡ抗体阳性率分别为19．6％和1．67％,22例CⅡ抗体阳性患者中20例可观测到阳性片段,其中最常见的特异位点抗体为CB10,CB11及D片段,其阳性率分别为45．4％,54．5％和45．4％。结论CB10,CB11及D片段出现与疾病严重程度有关。
Objective To investigate the clinical significance of type Ⅱ collagen fragments in rheumatoid arthritis （RA）. Methods Serum IgG antibodies were detected with enzyme linked immunosorbent assay（ELISA）. Positive sera were further analysed by western blot. Results The positive rates of IgG isotype in 112 patients with RA and 120 normal controls were 19.6% and 1.67% respectively. Twenty （90.9%） out of 22 positive patients were derected positive fragments by western blot, among which CB10, CB11 and D fragments were the predominant specific epitope antibodies, the positive rate was 45.4%, 54.5% and 45.4% respectively. Conclusions The main site specific antibodies found in patients are reactive to CB10, CB11 and D. Epitope specific antibodies are related to severity of RA.
出处
《中华风湿病学杂志》
CAS
CSCD
2006年第12期710-712,共3页
Chinese Journal of Rheumatology
基金
国家自然科学基金资助项目(39890857)
关键词
关节炎
类风湿
溴化氰
特异位点抗体
免疫印迹
Arthritis, rheumatoid
Cyanogen bromide
Specific epitope antibodies
Western blot
