摘要
目的探索β-连环蛋白促进毛囊干细胞增殖作用的分子机制。方法利用含有氨基端删除的β-连环蛋白的表达质粒转染毛囊细胞,观察转染细胞增殖,利用Westen blotting及原位杂交法观察其目的基因c-myc的表达。结果转染ΔN90β-连环蛋白基因后细胞集落形成率增加,细胞K19阳性率亦有所增加;转染细胞c-myc的表达在蛋白质水平与mRNA水平均增加。结论β-连环蛋白对毛囊细胞增殖分化的促进作用可能是通过促进c-myc基因的表达实现的。
Objective To study the mechanism of β-catenin on the regulation of prohferation and differentiation of hair follicle cells. Methods Amino-terminal truncated β-catenin gene was transfected into hair follicle cells. The expression of β- catenin was detected by immunocytochemistry to identify the success of transfection. Proliferation of transfected cells was measured by clone forming efficiency. The expression of keratin 19 of transfected cells was detected by immunocytochemistry. The expression of the target gene c-myc was observed by Western blotting and in situ hybridization. Results The expression of β-catenin in the nuclear after transfection increased indicating the success of amino-terminal truncated β-catenin gene transfection. Clone forming efficiency increased significantly in transfected cells, however, there was no significant increase of large clone. The expression of K19 increased slightly. Both of the expression of c-Myc protein and mRNA increased significantly after transfection, suggesting that the increase of β-catenin promoted the transcription and translation of c-myc gene. Conclusion The conclusion was drawn from these reults that the promotive effect of β-catenin on hair follicle cells＇ proliferation may be accomplished by increasing the expression of c-myc gene.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第6期660-664,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30170859)
高等学校博士点基金资助项目(20010023027)
