摘要
目的通过分析不同种类胆红素对三酰甘油(trig lyceride,TG)氧化酶测定方法的干扰,探讨直接胆红素(d irectb ilirub in,DB IL)、间接胆红素(ind irect b ilirub in IB IL)对过氧化氢酶偶联T rinder反应的TG氧化酶法测定结果的影响程度。方法制备系列不同浓度的DB IL、IB IL混合血清,且根据TG含量分为高、中、低三组,其中DB IL、IB IL均在日立7170A上检测;而每种血清标本中TG含量均经氧化酶法(GPO-PAP)和TG乙酰丙酮显色法检测。结果DB IL对TG检测的干扰大,当其浓度达280μm ol/L时,高、中、低三组标本中TG回收率仅为62.8%,60.3%,58.8%;而IB IL对TG检测的干扰小,当浓度达240μm ol/L时,TG回收率仍达90%以上。结论DB IL、IB IL对TG氧化酶测定结果的影响程度不同在实际操作中应区别对待。尤其在DB IL含量较高的标本用氧化酶法测定TG时产生的负干扰,在结果审核时应重视加以校正。
Objective To examine the effect of different kinds of bilirubin that include direct bilirubin and indirect bilirubin on the direct enzymatic measurement of triglyceride（TG） by using the TG and peroxidase couple reaction. Methods Prepared much mixed sera of the different level of direct bilirubin（DBIL）and indirect bilirubin （IBIL）,and they were divided three groups of high,middle and low according to the level of TG. The levels of DBIL and IBIL were measured by HITACHI 7170A automatic analyzer,the level of TG of each serum sample was measured by using both the direct enzymatic measurement and acetic acetone measurement. Results DBIL produced greatest negative interference,when concentration of DBIL was 280 μmol/L ,recovery rate of TG was only 62. 8% ,60.3% and 58. 8% ,but the effect of IBIL was slight ,when concentration was 240 μmol/L,recovery rate of TG was over 90%. Conclusion The DBIL and IBIL produce different interference in the enzymatic measurement of TG. They should be distinguished in every day work. Especially,the interference produced by the higher level of DBIL is greater,is adjusted in the result of checking.
出处
《现代检验医学杂志》
CAS
2006年第6期45-47,共3页
Journal of Modern Laboratory Medicine
关键词
直接胆红素
间接胆红素
三酰甘油
氧化酶法
direct bilirubin
indirect bilirubin
triglyeeride
enzymatic measurement
