摘要
五维同时荧光信息显微成像方法是一种新的荧光信息获取技术,它采用了双光子阵列点激发方式.这一方法可同时获取激发阵列点每点荧光的位置信息、荧光光谱信息和荧光寿命信息,弥补了现有荧光检测技术的不同功能信息不具有同时性的缺陷.给出了从这种技术的复合信息中提取复合光谱几何强度结构图像、不同光谱几何强度结构图像、不同光谱寿命图像的方法.提出了一种激发荧光强度修正系数矩阵方法,消除阵列点激发光强不均匀对激发荧光强弱产生的不利影响,取得明显效果.实验对实际样品做了数据采集和处理,给出图像结果,表明处理的效果良好.对存在的问题也作了讨论.
Microscopical imaging method of five-dimensional simultaneity fluorescence information is a new technology for acquiring fluorescence information, which makes use of two-photon array excitation to acquire information of fluorescence position, fluorescence spectrum and fluorescence lifetime for each array element. It removes the limitation on existing fluorescence detection technology in which different functional informations are not obtained simultaneously. In this paper, the methods for deriving geometrical intensity image, different spectral intensity images and different spectral life-time images from the complex information of the new technology are presented. Because of array excitation, there is asymmetry in the array excitation intensity, which influences the excited fluorescence intensity. A method based on the excitation fluorescence intensity correction coefficient matrix is put forward, which eliminates effectively the influence of the asymmetry of array excitation intensity, Data collection and processing for actual samples are made and imaging results are got. The experimental results show the validity of the processing methods and some unsolved problems in the processing methods are discussed.
出处
《物理学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第12期6701-6707,共7页
Acta Physica Sinica
基金
国家自然科学基金重点项目(批准号:60138010)资助的课题.~~
关键词
荧光信息处理
双光子
荧光光谱
荧光寿命
fluorescence information processing, two-photon, fluorescence spectrum, fluorescence lifetime
