摘要
探索了甘著叶片可溶性蛋白质的浸提方法,确定了蛋白质的沉淀方法,并分析了叶片可溶性蛋白粉中粗蛋白含量、粗纤维、粗脂肪和蛋白质中的氨基酸组成。结果表明,甘薯叶片主要含有一种分子量为53000Da的可溶性蛋白质,用pH为6．5～7．5的1％亚硫酸氢钠溶液浸提甘薯叶片粉末可较好地提取甘薯叶片中的可溶性蛋白质。经浸提、沉淀、透析、冻干处理获得的台农叶片蛋白粉中含有62．6％粗蛋白、0．18％粗纤维和0．51％粗脂肪。台农叶片可溶性蛋白质含有各种人体所需要的氨基酸,主要氨基酸是赖氨酸(99．88mg/g蛋白质)、亮氨酸(12026mg/g蛋白质)和天门冬氨酸(140 74mg/g蛋白质),并且必需氨基酸含量高于非必需氨基酸的含量,其比值为1．04,第一限制性氨基酸为色氨酸,氨基酸分数为75．9。
Extraction method for was studied and sweet potato leaf protein extraction method was optimized, and the contents of protein, fibre, lipid and amino acid in the precipitate were also analyzed, The results indicate that the major protein Mr from sweet potato leaf is 53000Da, The sweet potato leaf protein can be extracted well with 1% NaHSO3 （pH 6.5-7.5） solution. The protein powder of Tainong leaf contains 62,6% of protein, 0.18% of fibre and 0.51% of lipid and has full-spectrum amino acids, The main amino acids are Lysine （99.88mg/g protein）, Leucine （120.26mg/g protein）asparagine （140.74mg/g protein）, There were more essential amino acids than non-essential amino acid in the protein from Tainong leaf, and the ratio of them was 1,04. The first limiting amino acid was tryptophan, and the amino acid score was 75.9,
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第11期88-91,共4页
Science and Technology of Food Industry
基金
中国农业科学院与北京市大兴区科技合作项目资助
关键词
甘薯
叶蛋白
提取方法
成分分析
sweet potato
leaf protein
extraction method
composition analysis
