摘要
目的：建立检测患者全血中环孢素A（CsA）浓度的高效液相色谱法。方法：血样经乙醚-石油醚（70：30）萃取后，以C18硅胶键合相为固定相，乙腈-甲醇（80：20）为流动相，70℃柱温下进行色谱分析，紫外检测波长为210nm。结果：CsA全血浓度在50-1600ng／mL范围内呈良好的线性关系，r=0．9998，方法的平均回收率为97．6％，日内和日间精密度RSD均小于6％（n=5），最低检测限为10ng／mL。结论：本法简便、快速，易于开展，用于肾移植术后患者CsA的血药浓度监测，效果良好。
Objective：To establish a practical RP-HPLC method to determine the concentration of eyclosporin A in patient＇s whole Hood. Methods： CsA was extracted from whole blood with ether and separated, using a C18 column and a mobile phase of acetonitrile-methanol （80：20）. The column temperature was maintained at 70 ℃ and CsA was detected at 210 nm. Results： A good linear relationship was obtained between the concentration of CsA from 50 ng/mL to 1 600 ng/mL, r = 0.9998. The average recovery rate of CsA was 97.6%, and the RSD within a day and between days were less than 6% （n = 5）. The detection limit of CsA was 10 ng/mL. Conclusions： The established method was accurate, simple, sensitive and suitable for the therapeutic monitoring of CsA.
出处
《儿科药学杂志》
CAS
2006年第6期3-4,7,共3页
Journal of Pediatric Pharmacy
